Protein Info for LRK53_RS16490 in Rhodanobacter sp000427505 FW510-R12
Annotation: phosphoenolpyruvate carboxylase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 62% identical to CAPP_XANOR: Phosphoenolpyruvate carboxylase (ppc) from Xanthomonas oryzae pv. oryzae (strain KACC10331 / KXO85)
KEGG orthology group: K01595, phosphoenolpyruvate carboxylase [EC: 4.1.1.31] (inferred from 62% identity to psu:Psesu_0497)Predicted SEED Role
"Phosphoenolpyruvate carboxylase (EC 4.1.1.31)" in subsystem Fermentations: Mixed acid or Pyruvate metabolism I: anaplerotic reactions, PEP (EC 4.1.1.31)
MetaCyc Pathways
- mixed acid fermentation (13/16 steps found)
- nitrogen remobilization from senescing leaves (7/8 steps found)
- partial TCA cycle (obligate autotrophs) (7/8 steps found)
- CO2 fixation into oxaloacetate (anaplerotic) (2/2 steps found)
- ethene biosynthesis V (engineered) (18/25 steps found)
- C4 photosynthetic carbon assimilation cycle, NAD-ME type (7/11 steps found)
- C4 photosynthetic carbon assimilation cycle, NADP-ME type (4/7 steps found)
- C4 photosynthetic carbon assimilation cycle, PEPCK type (8/14 steps found)
- formaldehyde assimilation I (serine pathway) (6/13 steps found)
- gluconeogenesis II (Methanobacterium thermoautotrophicum) (8/18 steps found)
- Methanobacterium thermoautotrophicum biosynthetic metabolism (25/56 steps found)
KEGG Metabolic Maps
- Carbon fixation in photosynthetic organisms
- Pyruvate metabolism
- Reductive carboxylate cycle (CO2 fixation)
Isozymes
No predicted isozymesUse Curated BLAST to search for 4.1.1.31
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (901 amino acids)
>LRK53_RS16490 phosphoenolpyruvate carboxylase (Rhodanobacter sp000427505 FW510-R12) MEASREPEFLPPDGPLREDVGRLGAMVGRMLAEQGGRAFFERVEQVRQAAIRRRREGATL EELAASLAGLDARDAEALARAFATYFQAVNTAERVHRIRRRRDYQREGSAPQPESLLDVL GRLKAEGVGADELVGWLDRLWIEPVFTAHPTEAVRRSLLEKEQAIVASLIDGFDRERTPQ ERKDDDDRIYMALSAGWQTAEASPVRPSVLDEREHVDFYLAHPLYRIVPTLYESLAQALQ TTYGVAIRLPRLLRFASWVGGDMDGNPNVGADTIADCLDSQRALVLERYREDVAALARSL SQTEGRVAASDALRVRLADYRARFPAAAAQIRPRHADMPYRCLLQLVGARLALTQDDDAD GYASSDEFLDDLQLIADSLFQHRGVHAGAYAVERLQCRVRSFGFHLARLDVRQDSRVHDD ALAALLAEPDWATRDGAARADRLRPYASGDAVFPDSHDANATSLQAVFAILRDSRKSHGC DATGLYIISMARSAADVLAVLALARRGGLVDPANDVPLNIAPLFETVDDLKSAPATLRAL LDDPVYRRHLAARGDQQWVMLGYSDSGKDGGTLASRWGLQRAQVELLEVAHAAGIQLAFF HGRGGSASRGGARITPALMSAPRGAVAGVLRVTEQGEVIHRKYGIRALALRNLEQTVGAV LRASLRPREDESREERWREVMNTLSSTSRQSYRAFVERDGFVDYFRSATPVDVIERMTLG SRPASRRSMRGVQDLRAIPWVFAWTQCRSILPGWYGLGSALEQGARQFGEETLVEMARDW AFFCNLLDDVEMVLAKCDLPIAEAFSKLSGPLHEEFFGLVRDEFARTRRWLLQLKGSGVL LRGEPRLAASIRLRNPYVDPMSLLQVDLLQRWRASDRQDDAVLQALVACVNGVAQGLQNT G