Protein Info for LRK53_RS14980 in Rhodanobacter sp000427505 FW510-R12

Annotation: chemotaxis protein CheA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 634 PF01627: Hpt" amino acids 8 to 99 (92 residues), 57 bits, see alignment E=3.7e-19 PF02895: H-kinase_dim" amino acids 243 to 302 (60 residues), 54.6 bits, see alignment 2.5e-18 PF02518: HATPase_c" amino acids 350 to 489 (140 residues), 66 bits, see alignment E=8.1e-22 PF01584: CheW" amino acids 495 to 617 (123 residues), 74.5 bits, see alignment E=1.3e-24

Best Hits

KEGG orthology group: K03407, two-component system, chemotaxis family, sensor kinase CheA [EC: 2.7.13.3] (inferred from 53% identity to psu:Psesu_1477)

Predicted SEED Role

"Signal transduction histidine kinase CheA (EC 2.7.3.-)" in subsystem Bacterial Chemotaxis or Flagellar motility or Two-component regulatory systems in Campylobacter (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (634 amino acids)

>LRK53_RS14980 chemotaxis protein CheA (Rhodanobacter sp000427505 FW510-R12)
MDAELDNELRQDFLVEAGELLQRLDEQLVGLEAAPGDGELLNAVFRAFHTVKGGAGFLAL
EPMVLLCHHAEDLLNEARNGKLVLGAVQMDALLHALDLLNGMMAAVGAAAPLQMPPPALL
AALLPEARPVAVAAPAPPVAAAGGSIDDSEFEALLDSMYGNAAPGAVAPAPLPAAPPASA
TRTIDDDEFEALLDTLHGKPGQPAAPMPAPVAVPAPAAPGTPANAATTTPAAAPAHHAAP
VENSVRVDTARLDLLVNHAGELVLLRNRLLSLAARHGSEALAAAANELDRVTDELQGAVM
GMRMQPVGRLFQRFPRIVRDLARQLGKDVELVLEGEGTDLDRGLVEALADPLIHLLRNAL
DHGVELPAERERAGKPRKGRVCLSASQRGERIVIAVRDDGRGMDPEILRRKAVEKGVIDA
VQAARLSEAECYELIFRPGFSTAAVVSDISGRGVGMDVVKTRVAELGGTLQVRSQLGHGS
ELELTVPLTLAVLRVLMVRVDTRLFALPLGNVAEVFELDAGQDCLLDGRLVARHRGRALP
LLDLTGWAGAPAAAGRHVVVLHIGHRRLGCLVHEVLGREDVIVKPLGPLFGGVPGIAGAT
VTGDGRLALVMDLASLAGLAGDDDQPSTSLQSTG