Protein Info for LRK53_RS14535 in Rhodanobacter sp000427505 FW510-R12

Annotation: nitric-oxide reductase large subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 760 signal peptide" amino acids 1 to 32 (32 residues), see Phobius details transmembrane" amino acids 230 to 249 (20 residues), see Phobius details amino acids 284 to 305 (22 residues), see Phobius details amino acids 334 to 353 (20 residues), see Phobius details amino acids 365 to 386 (22 residues), see Phobius details amino acids 412 to 433 (22 residues), see Phobius details amino acids 445 to 466 (22 residues), see Phobius details amino acids 481 to 504 (24 residues), see Phobius details amino acids 516 to 539 (24 residues), see Phobius details amino acids 545 to 566 (22 residues), see Phobius details amino acids 587 to 609 (23 residues), see Phobius details amino acids 629 to 648 (20 residues), see Phobius details amino acids 667 to 688 (22 residues), see Phobius details amino acids 723 to 741 (19 residues), see Phobius details PF22085: NorB_cytochrome_c-like" amino acids 37 to 217 (181 residues), 234.5 bits, see alignment E=1.1e-73 PF00115: COX1" amino acids 280 to 723 (444 residues), 195.6 bits, see alignment E=1.5e-61

Best Hits

KEGG orthology group: K04561, nitric oxide reductase, cytochrome b-containing subunit I [EC: 1.7.2.5] (inferred from 70% identity to psu:Psesu_0363)

MetaCyc: 51% identical to nitric oxide reductase (menaquinol) (Cupriavidus necator H16)
RXN-12129 [EC: 1.7.5.2]

Predicted SEED Role

"Nitric-oxide reductase (EC 1.7.99.7), quinol-dependent" in subsystem Denitrification or Flavohaemoglobin or Nitrosative stress (EC 1.7.99.7)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.2.5, 1.7.99.7

Use Curated BLAST to search for 1.7.2.5 or 1.7.5.2 or 1.7.99.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (760 amino acids)

>LRK53_RS14535 nitric-oxide reductase large subunit (Rhodanobacter sp000427505 FW510-R12)
MSTTRKLWLGLAALLVASFAVLLWVGSVVHQQAPPLPTAVVTGDGQTLYTKADVEEGRQV
WQSIGGQQLGSIWGHGALLAPDWSADWLHRESMAMLELLSRDAGLGPYDSLTAQQQAPLK
ARVQEELRTNTWDAASNTITVSALRAHAMEAVAAHYMSLFGNDPATHKLREGYAMRENTV
AEMEHRREVTAFFWWTSWAAATQRPNEAMSYTQNWPYEPLAGNTPTSTSFLWSVFSILFM
IFGIGLLGWHHARQVSHEPLPTVPAHDPLTEFKATPSMKATGKYFWTVLGLFLAQILLGA
TTAHYQVEGQQAYGFALANYLPYSLTRTWHTELAVLWIATAWLATGLYIAPLISGHEPKF
QRLGVNFLWVCLLVIVVGSFAGQWFAVMDKMGLQYNFWFGHQGWEYTDLGRFWQIFLFIG
LMLWLFLVGRALWPAMDRNKEGASIVGLLFLSTVAIGLLYGAGLMWGEHTHIAMVEYWRW
WVVHLWVEGFFEVFATAVISYLFVRLGLLRVTTATTNVLFATIVFMAGGVLGTLHHLYFS
GTTTAVIALGASFSALEVVPLALIGLEAHDTWKKQHAAPWMARYRWPIMFFVAVSFWNLV
GAGLLGFLVNTPLALYYMQGLNLTATHGHTALFGVYGMLGLGLMLFCLRGIKPQAEWREG
WLRGSFWLLNIGLSMMAVLTLLPLGILQLKAVLEHGYWFARSAEFMDRPLIHMLVWMRVP
GDTLFAVGALLIAVFVAALWLRPKRRTAGAPLSQGVPERA