Protein Info for LRK53_RS13665 in Rhodanobacter sp000427505 FW510-R12

Annotation: 3-hydroxyacyl-CoA dehydrogenase NAD-binding domain-containing protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 716 transmembrane" amino acids 660 to 682 (23 residues), see Phobius details PF00378: ECH_1" amino acids 14 to 202 (189 residues), 130 bits, see alignment E=2.6e-41 PF16113: ECH_2" amino acids 17 to 191 (175 residues), 70.7 bits, see alignment E=4e-23 PF02737: 3HCDH_N" amino acids 317 to 495 (179 residues), 194 bits, see alignment E=5.5e-61 PF00725: 3HCDH" amino acids 498 to 598 (101 residues), 55.8 bits, see alignment E=1.3e-18

Best Hits

KEGG orthology group: K01782, 3-hydroxyacyl-CoA dehydrogenase / enoyl-CoA hydratase / 3-hydroxybutyryl-CoA epimerase [EC: 1.1.1.35 4.2.1.17 5.1.2.3] (inferred from 78% identity to bur:Bcep18194_C7186)

Predicted SEED Role

"Enoyl-CoA hydratase [isoleucine degradation] (EC 4.2.1.17) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3)" in subsystem Acetyl-CoA fermentation to Butyrate or Isoleucine degradation or Polyhydroxybutyrate metabolism or Valine degradation or n-Phenylalkanoic acid degradation or Isobutyryl-CoA to Propionyl-CoA Module (EC 1.1.1.35, EC 4.2.1.17, EC 5.1.2.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.35, 4.2.1.17, 5.1.2.3

Use Curated BLAST to search for 1.1.1.35 or 4.2.1.17 or 5.1.2.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (716 amino acids)

>LRK53_RS13665 3-hydroxyacyl-CoA dehydrogenase NAD-binding domain-containing protein (Rhodanobacter sp000427505 FW510-R12)
MTANNPVTLEIDADGIGLVTFDQPGRAMNVLNPELVAPFAAIVERLEKEEAIKGLVLTSG
KSTFIVGADIDQLTAISTAEEAFSLCEDLKALLRRIEKCGKPVVAALNGTALGGGLEVAL
ACHARFALDEAALKLGLPEVKLGLLPGGGGTQRLPRMIGIQKSFELLTQGTELRAAEARG
LGLVNDLATSREELLQKSRAWCVANPRAAQPWDKQGFRIPGGDSKHPGVVQLLAIAPSIA
NAKAHGNYPAIGHIMSCLFEGCLLDFDAACQVESRYFAACVVSQESRNMIGTLWHQLNAI
KKGQSRPAGVAPCRVRKIGILGAGMMGAGIAYVSAKAGIEVILLDTTIENAEKGKSYSQG
LLNKAILRGRSTPDKRDALLAKITPTTRYEDLQGCDLVIEAVFEDRAIKAACTQKAEAVI
AADAVFASNTSTLPITGLAKASVRPKNFIGLHFFSPVDKMPLVEIIVGEQTSNETLARGF
DYVLQIGKTPIVVNDSRGFYTSRVFATYVMEGLAMLGEGVHPRSIEAAGIKAGMPMPPLA
LQDEVSLGLSLHVADQTRKDLAAEGKPLPEHPGEPVLRMIGGTHQRLGRKTGKGLYDYDG
RDKHLWPELTRLYPTAAEQPTQQELIDRLMFVQANEAARCFEENVVRSVADANIGSIFGW
GFAPFHGGALQFISAMGAAGFVARSRELAERYGARFAPADIVVKQAAVGGRFEGAA