Protein Info for LRK53_RS13630 in Rhodanobacter sp000427505 FW510-R12

Annotation: alanine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00344: alanine--tRNA ligase
  • amino acids 5 to 855 (851 residues), 1027.1 bits, E=0
• PF01411: tRNA-synt_2c
  • amino acids 5 to 556 (552 residues), 727.9 bits, E=1.2e-222
• PF07973: tRNA_SAD
  • amino acids 654 to 697 (44 residues), 64.6 bits, 9.4e-22
• PF02272: DHHA1
  • amino acids 772 to 873 (102 residues), 56.7 bits, E=4.4e-19

Best Hits

Swiss-Prot: 70% identical to SYA_STRMK: Alanine--tRNA ligase (alaS) from Stenotrophomonas maltophilia (strain K279a)

KEGG orthology group: K01872, alanyl-tRNA synthetase [EC: 6.1.1.7] (inferred from 70% identity to sml:Smlt1743)

MetaCyc: 62% identical to alanine--tRNA ligase/DNA-binding transcriptional repressor (Escherichia coli K-12 substr. MG1655)
Alanine--tRNA ligase. [EC: 6.1.1.7]; 6.1.1.7 [EC: 6.1.1.7]; 6.1.1.7 [EC: 6.1.1.7]; RXN-19726 [EC: 6.1.1.7, 3.1.1.96]; 3.1.1.- [EC: 6.1.1.7, 3.1.1.96]

Predicted SEED Role

"Alanyl-tRNA synthetase (EC 6.1.1.7)" (EC 6.1.1.7)

MetaCyc Pathways

• tRNA charging (21/21 steps found)
• shinorine biosynthesis (3/8 steps found)
• superpathway of chorismate metabolism (39/59 steps found)
• vanchrobactin biosynthesis (2/8 steps found)
• ergotamine biosynthesis (4/12 steps found)
• (2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)
• bacillibactin biosynthesis (3/12 steps found)
• enterobactin biosynthesis (2/11 steps found)
• superpathway of ergotamine biosynthesis (5/18 steps found)
• sulfazecin biosynthesis (1/16 steps found)
• phosalacine biosynthesis (4/25 steps found)
• phosphinothricin tripeptide biosynthesis (4/25 steps found)
• corallopyronin A biosynthesis (2/30 steps found)
• colibactin biosynthesis (7/38 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Aminoacyl-tRNA biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.1.96 or 6.1.1.7

Protein Sequence (875 amino acids)

>LRK53_RS13630 alanine--tRNA ligase (Rhodanobacter sp000427505 FW510-R12)
MKTSDIRSAFLDYFRSKGHTIVPSSSLVPASDPTLLFTNSGMVPFKNVFLGSEKLPYVRA
ADVQRCLRAGGKHNDLDAVGYTARHHTFFEMLGNWSFGDYFKREAITWAWELLTGVFKLP
AEKLWVTVYHTDDEAFDIWHKQVGVPAERIVRIGDNKGAPYASDNFWQMADTGPCGPCTE
IFYDHGPGVAGGPPGSPDEDGDRYIEIWNLVFMQFDRAPDGTLSPLPAPCVDTGMGLERL
AAVLQHVHSNYEIDLFAHLIRVAGELTGTKDLANKSLRVIADHIRACAFLIVDGVLPSNE
GRGYVLRRIIRRALRHGWMLGVRGDFFWKMVQPLVEEMGQAYPELPAKQAFVEDALRTEE
HRFGETLEHGMRLFDEVAAKSGRIIPGADAFRLYDTYGFPVDLTADIARERGLEVDMAGF
EQAMNEQRERARAAGKFEAKGQMPAELASQLKPTVFLGYEALHGSGSKVLGIVRGGKQVE
QLGKGEQGFVILDRTPFYAESGGQVGDTGMLSNPAGRFEVDDTLKMGGVFFGHAGTWHGA
QPLKAGDRVDAQVDAARRQAIVLNHSATHLLHAALRQVLGTHVAQKGSLVAPERLRFDFS
HRKPMSHDELVRVEALVNAEVRRNAAAEVRHMAYDDAIAFGAMALFGEKYGDEVRVLKMG
DFSTELCGGTHVGRTGDIGLFKIVSEAGVASGVRRIEAVTGTGALAWVADEERRLTEFSQ
LLSSNGDDAVEKLRQLFERQRKLERELESLRSKAAGSATADLAGSAKDVDGIKVVAARLE
GLDAKSLRDSVDQLKQQLGDCVVLLAGAAEGRVSLIAGVHGKALARVKAGSVVAHVAGQI
DGKGGGRPDMAQGGGTDAPNLPAILSSLPDWISAQ