Protein Info for LRK53_RS12690 in Rhodanobacter sp000427505 FW510-R12

Annotation: M4 family metallopeptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 515 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF07504: FTP" amino acids 67 to 102 (36 residues), 32.1 bits, see alignment 1.2e-11 PF01447: Peptidase_M4" amino acids 227 to 365 (139 residues), 112.8 bits, see alignment E=3e-36 PF02868: Peptidase_M4_C" amino acids 368 to 512 (145 residues), 114.6 bits, see alignment E=6.9e-37

Best Hits

Predicted SEED Role

"Vibriolysin, extracellular zinc protease (EC 3.4.24.25) @ Pseudolysin, extracellular zinc protease (EC 3.4.24.26)" (EC 3.4.24.25, EC 3.4.24.26)

Isozymes

Compare fitness of predicted isozymes for: 3.4.24.25, 3.4.24.26

Use Curated BLAST to search for 3.4.24.25 or 3.4.24.26

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (515 amino acids)

>LRK53_RS12690 M4 family metallopeptidase (Rhodanobacter sp000427505 FW510-R12)
MMHKPLAAALLGAFGLAAMSDAGAATHTRVQAQTMGQIAPAALASRIGLEADASLSPRLQ
ARTAHGTFKTREQQTFRGVPVYGRQVVVERDGSGSVLGVSGEISRGISHDVASVAPAING
ARATALLREHAGLLPLGIGVNGIQDSDIQNAKNDLYVYAEGNRARLAYLSSFFVDRGNGP
TRPMAIIDANTGEVIQAWEGLTTKGKPGAGTVTGTPALATGAGGNLKTGQYFYGTNYASL
AVTQSGSTCYMQNTNVKTNNMAGSTRTGTLWSFICPNSSGDGVNGAYSPINDAHHFGGVV
HDMYNTWLGAPPLTFSLVMNVHYGRNYENAFWNGSSMNFGDGASYFYPLTSLDVTSHEVS
HGFTEQHSNLQYSGQSGGINEAFSDMAGEAAEFYDRGGNDWLVGADIMKTGTALRWMCTP
TQDGRSIDNAANFTSSMDVHYSSGVYNKAFCTLAKTTNWNTKKAFEVFARANAMYWTATS
TFNSGACGVETAAVDYAYTRSDVTAAFNAVGVTCK