Protein Info for LRK53_RS09730 in Rhodanobacter sp000427505 FW510-R12

Annotation: ATP-dependent Clp protease ATP-binding subunit ClpA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 756 PF02861: Clp_N" amino acids 1 to 126 (126 residues), 94.9 bits, see alignment E=2.8e-30 TIGR02639: ATP-dependent Clp protease ATP-binding subunit ClpA" amino acids 3 to 735 (733 residues), 1075.1 bits, see alignment E=0 PF00004: AAA" amino acids 212 to 325 (114 residues), 46.4 bits, see alignment E=3.3e-15 amino acids 493 to 608 (116 residues), 42.5 bits, see alignment E=5.3e-14 PF17871: AAA_lid_9" amino acids 352 to 453 (102 residues), 88.5 bits, see alignment E=1.5e-28 PF07724: AAA_2" amino acids 487 to 648 (162 residues), 194.1 bits, see alignment E=1.1e-60 PF13401: AAA_22" amino acids 490 to 583 (94 residues), 27.3 bits, see alignment E=2.5e-09 PF00158: Sigma54_activat" amino acids 492 to 608 (117 residues), 22 bits, see alignment E=7e-08 PF07728: AAA_5" amino acids 492 to 608 (117 residues), 47.4 bits, see alignment E=1.3e-15 PF10431: ClpB_D2-small" amino acids 654 to 733 (80 residues), 84.4 bits, see alignment E=2.8e-27

Best Hits

Swiss-Prot: 64% identical to CLPA_ECO57: ATP-dependent Clp protease ATP-binding subunit ClpA (clpA) from Escherichia coli O157:H7

KEGG orthology group: K03694, ATP-dependent Clp protease ATP-binding subunit ClpA (inferred from 77% identity to psu:Psesu_1536)

MetaCyc: 64% identical to ATP-dependent Clp protease ATP-binding subunit ClpA (Escherichia coli K-12 substr. MG1655)
Endopeptidase Clp. [EC: 3.4.21.92]

Predicted SEED Role

"ATP-dependent Clp protease ATP-binding subunit ClpA" in subsystem Proteolysis in bacteria, ATP-dependent

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.92

Use Curated BLAST to search for 3.4.21.92

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (756 amino acids)

>LRK53_RS09730 ATP-dependent Clp protease ATP-binding subunit ClpA (Rhodanobacter sp000427505 FW510-R12)
MFSKDLEVTIGHCYKRAREQRHEFMTVEHLLLALTENQSALGALRACGADLPRLSADLER
IINETVPVLPPGDERDTQPTLGFQRVLQRAVYHVQSSGRKEVTGANVLVAIFGEKDSHAV
YFMHQQEITRLDVVNYISHGIAKIGDEPAAGVSGGEREGEEGGEPKGNPLHEFASNLNEL
ALEGKIDPLIGRADEIERTIQVLCRRRKNNPLYVGEAGVGKTALAEGLAKRIVDGEVPEV
LESATIWSLDLGALVAGTKYRGDFEKRLKGVIAQLKKQPGAILFIDEIHTIIGAGSASGG
TMDASNLIKPMLASGELRCIGSTTFQEYRGVFEKDRALARRFQKIDVVEPTVADSIEILK
GLRSRFEEHHHVAYTNEALKAAVDLSVKHIPDRLLPDKAIDVIDEAGARQRLLPEDQRTG
KVDVGEVEYIVAKMARIPAKQVSASDRDVLRNLERNLKMVVFGQDPAIEALAASIKMARS
GLADPSKPIGCFLLAGPTGVGKTEVTKQLAMQLGIEMIRFDMSEYMEAHSVSRLVGAPPG
YVGFDQGGLLTEAVTKHPHAVLLLDEIEKAHPDVFNILLQVMDRGVLTDTNGREANFKNV
VVVMTTNAGAAIAARRSMGFVEQKHESDAMEVIRRIFTPEFRNRLDAIIQFGALDFEHIL
RVVDKFLIELESQLTEKRVSLDVGADARRWLAEHGFDPQMGARPMARVIQEKVKRALADE
LLFGKLAEGGVVRLSVADGELKVDCKAAEKLPAVVE