Protein Info for LRK53_RS09490 in Rhodanobacter sp000427505 FW510-R12

Annotation: glycine C-acetyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 399 TIGR01822: glycine C-acetyltransferase" amino acids 7 to 397 (391 residues), 728.6 bits, see alignment E=8.2e-224 PF00155: Aminotran_1_2" amino acids 43 to 386 (344 residues), 223 bits, see alignment E=6.8e-70 PF01212: Beta_elim_lyase" amino acids 90 to 326 (237 residues), 27.6 bits, see alignment E=1.9e-10

Best Hits

Swiss-Prot: 65% identical to KBL_SALTY: 2-amino-3-ketobutyrate coenzyme A ligase (kbl) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K00639, glycine C-acetyltransferase [EC: 2.3.1.29] (inferred from 82% identity to psu:Psesu_2271)

MetaCyc: 65% identical to 2-amino-3-ketobutyrate CoA ligase (Escherichia coli K-12 substr. MG1655)
Glycine C-acetyltransferase. [EC: 2.3.1.29]

Predicted SEED Role

"2-amino-3-ketobutyrate coenzyme A ligase (EC 2.3.1.29)" in subsystem Glycine Biosynthesis or Glycine and Serine Utilization (EC 2.3.1.29)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.29

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (399 amino acids)

>LRK53_RS09490 glycine C-acetyltransferase (Rhodanobacter sp000427505 FW510-R12)
MSYSAKARYASELDAIREQGLFKAERIITSPQSAEIELQGGRRVLNFCANNYLGLADHPA
VIQAAKDALDSHGFGMASVRFICGTQDLHKQLEAKIAEFFGTEDTILYAACFDANGGLFE
PLLGEQDAVISDALNHASIIDGIRLCKAKRFRYANSDMADLEQQLQAADAAGVRTKLIST
DGAFSMDGFIARLDQITALAAKYDAMVHIDECHCTGFLGDSGRGSAEVNGVMDKIDIFTG
TLGKALGGALGGFTTGRREVIELLRQRSRPYLFSNSLPPHVVAAAIKVFDMLSSAGELRE
KLKENTRYFRERMTAAGFDIKPGVHPIVPVMIYDAPKAQAMAAALLEEGIYVTGFFYPVV
PQGQARIRTQMSAAHTREHLDRAIAAFTKVGRKLGVIGG