Protein Info for LRK53_RS09445 in Rhodanobacter sp000427505 FW510-R12

Annotation: alkyl hydroperoxide reductase subunit F

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 528 TIGR03140: alkyl hydroperoxide reductase subunit F" amino acids 1 to 517 (517 residues), 802.6 bits, see alignment E=6.9e-246 PF13192: Thioredoxin_3" amino acids 124 to 194 (71 residues), 32.4 bits, see alignment E=2.8e-11 PF07992: Pyr_redox_2" amino acids 212 to 504 (293 residues), 162.7 bits, see alignment E=4.7e-51 PF01134: GIDA" amino acids 213 to 246 (34 residues), 19.4 bits, see alignment (E = 1.8e-07) PF13738: Pyr_redox_3" amino acids 303 to 487 (185 residues), 45.1 bits, see alignment E=3e-15 PF00070: Pyr_redox" amino acids 357 to 431 (75 residues), 46.8 bits, see alignment E=1.2e-15

Best Hits

Swiss-Prot: 80% identical to AHPF_XANCH: Alkyl hydroperoxide reductase subunit F (ahpF) from Xanthomonas campestris pv. phaseoli

KEGG orthology group: K03387, alkyl hydroperoxide reductase subunit F [EC: 1.6.4.-] (inferred from 80% identity to xcc:XCC0833)

MetaCyc: 65% identical to alkyl hydroperoxide reductase, AhpF component (Escherichia coli K-12 substr. MG1655)
RXN-8506 [EC: 1.5.1.37]; R4-RXN [EC: 1.5.1.37, 1.11.1.26]

Predicted SEED Role

"Alkyl hydroperoxide reductase protein F (EC 1.6.4.-)" in subsystem Thioredoxin-disulfide reductase (EC 1.6.4.-)

Isozymes

Compare fitness of predicted isozymes for: 1.6.4.-

Use Curated BLAST to search for 1.11.1.26 or 1.5.1.37 or 1.6.4.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (528 amino acids)

>LRK53_RS09445 alkyl hydroperoxide reductase subunit F (Rhodanobacter sp000427505 FW510-R12)
MLDANLSTQLKAYLEKVTQPIEIVASLDDSAKSAELNELLEQIASLSDRISLVRRDDDAR
KPSFAINRVGSDIGVRFAGIPLGHEFTSLVLALLQVGGHPSKAAAEVIEQVRNLDGDYQF
ETYFSLSCQNCPDVVQALNLMSVLNPKIQHVAIDGALYQGEVEARQVMSVPTVYLNGELF
DQGRMSLEQILAKLDTGASVREAEKIKAKGAFDVLVVGGGPAGAAAAIYAARKGIRTGVA
AERFGGQVLDTMAIENFISVPYTEGPKLGAALEQHVRDYDVDVMNLQRAEKLVPASDATG
GLVEIRLANGATLRSKTVILSTGARWRQMGVPGEEEYRNKGVAYCPHCDGPLFKGKRVAV
IGGGNSGVEAAIDLAGLVAHVTLVEFDGRLRADEVLQRKLRSLSNVDIVVSAQSTEVLGD
GQRVTGLVYKDRTDGMMHSLALEGIFVQIGLLPNTEWLKGTLELSPRGEIVIDARGRTSL
PGVFAAGDATTVPYKQIVIAMGAGSTAALSAFDHLIRSPAPVAKTVAA