Protein Info for LRK53_RS07945 in Rhodanobacter sp000427505 FW510-R12

Annotation: NAD-glutamate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF21075: GDH_ACT1
  • amino acids 36 to 167 (132 residues), 102 bits, E=1e-32
• PF21073: GDH_HM1
  • amino acids 336 to 395 (60 residues), 80.3 bits, see alignment (E = 3.1e-26)
• PF21076: GDH_ACT2
  • amino acids 399 to 488 (90 residues), 99.8 bits, 4.4e-32
• PF21079: GDH_HM2
  • amino acids 494 to 538 (45 residues), 46.6 bits, see alignment (E = 1.1e-15)
• PF21077: GDH_ACT3
  • amino acids 544 to 613 (70 residues), 66 bits, see alignment (E = 8.4e-22)
• PF21078: GDH_HM3
  • amino acids 635 to 700 (66 residues), 104.4 bits, see alignment (E = 1.1e-33)
• PF05088: Bac_GDH_CD
  • amino acids 763 to 1254 (492 residues), 864.2 bits, E=1e-263
• PF21074: GDH_C
  • amino acids 1299 to 1634 (336 residues), 389.2 bits, E=6.9e-120

Predicted SEED Role

"NAD-specific glutamate dehydrogenase (EC 1.4.1.2), large form" in subsystem Glutamate dehydrogenases or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.1.2)

MetaCyc Pathways

• L-alanine degradation II (to D-lactate) (3/3 steps found)
• L-glutamate degradation I (1/1 steps found)
• ethene biosynthesis IV (engineered) (1/3 steps found)
• L-glutamate degradation V (via hydroxyglutarate) (5/10 steps found)
• methylaspartate cycle (11/19 steps found)
• L-glutamate degradation XI (reductive Stickland reaction) (2/7 steps found)
• 4-aminobutanoate degradation V (1/7 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Glutamate metabolism
• Nitrogen metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.1.2

Protein Sequence (1641 amino acids)

>LRK53_RS07945 NAD-glutamate dehydrogenase (Rhodanobacter sp000427505 FW510-R12)
MNATPAANDPSLSSVVFAELKRGGFSPERLSEAQVFCEAFFARVGGGDAHLHTPVQWAAL
VGSLLEFMQKRREGQACVRVLSPADVQAGRSLLQIVTDDMPFLVDTVSMVVSAKLQIHVV
IHPALKVARDASGKLLGLGGAAGSAESLMHFEIDRVADAAEQAQLEGQVAAALEDVRAAV
GDWSAMRDKALAVANQLPQRKLPLDTASVQEASEFMRWIAADNFTFLGYREYEVADADGD
RVLRAVESSGLGILRSNERSMAPRSLSTLAASELPQSGAIDAIILTKTNARSRVHRTGYM
DYIGVLQFGADGRAVAEQRFLGLFSSNAYMARPQDVPLVRHKAEAVLARSGLKRDSYSGR
SLRHILETLPREELFQSTEDELFATAMGILELRQRARTRLFMRRDRYGRFFTCMVFVPRD
RFNTSVRERIESLLRTALHAEQSDSSVQMGEAVLARLTIVLRPKIGDHPSYDLAELEQGV
ASIVRNWHDEVRDALVRLRGEHEGVVLANRYARALPAGYIEDVSPAVAAEDVYQLSQLVG
DNALRMSFYHPPKAPETLRFKVYRSGGDIALSEVLPQLENLGLRVLTEHVYEVSGEAPLS
IQDFEVQPVGKLTFGVEQVGALFEDAFEQIWRGNAENDGFNRLVLGAKLSWRQVAMLRGY
CKYLLQTGATFSQSYMEETLNRYPAIAGLLVELFLAKFDPRREALSADELQAAGATLAAE
MLALIPGNVQAAQPGLIGELAGSLSKPRDEQVQAVESLVGILLENVASLDEDRILRGFVK
LIRATLRTSFFQQWDGAYRDYISYKLDSHAVPGLPKPVPYREIWVCAPRVEGIHLRFGAV
ARGGLRWSDRREDFRTEVLGLVKAQMVKNTVIVPVGSKGGFFVKKSPAASDRDAVLAEGI
ACYRLFINGLLDVTDNLVDGKVVHPHDVVRHDADDPYLVVAADKGTAKFSDIANAISIEH
NYWLGDAFASGGSHGYDHKGMGITARGAWESVKRHFRALGRDCQTQDFTCVGIGDMSGDV
FGNGMLLSEHTLLVAAFDHRHVFLDPNPDAARSFVERKRMFDVPRSSWDDYDKSLISAGG
GIYPRSAKSIPLSPEMRAVLGLKPEVEQLAPSDLLSAILKAPVDLIFNGGIGTYIKSSGE
THAEVSDRANNALRINGADVRAKIIGEGGNLGMTQKGRIEAAQHGVLLNTDFIDNSAGVD
TSDHEVNIKILLNDAVQRGELGFDARNAQLAAMTDEVAQLVLWDNYRQNQAITLMEHQSV
HRIGSMAHFIRTLEAEGTLDRQVENLPGEAELTERKSRGLGLTRPELAVLLSYDKIRLFQ
QLLDSDVPEDPYLSKELVRYFPLPLHETYAAHMQRHRLKREIIATAVTNSTINRMGATFM
MRMQEDTGQGPAAIAKAYTAAREILEARELWAEIEALDAKVAEDTQIDAIKQIWSLLRHM
TRWLLNRPGGSLDIAANVERYQAGVSALRKALPEVLTATGKGDFSSSQEKWEGLGLPAEL
ALRMARLPELRAALDMVEVSQQSGQSIEKVAGVFFELGEALDLEWLRDQIEALPVEGHWH
AQARGSLLDELNHQHRALALQVLSLGGGSRDISPVQAWLQRDDATLQYTRGMLAEILTQN
ADYPIASVAVRRLAQLAQVPV