Protein Info for LRK53_RS07685 in Rhodanobacter sp000427505 FW510-R12

Annotation: acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 665 PF00289: Biotin_carb_N" amino acids 1 to 110 (110 residues), 158.2 bits, see alignment E=4.4e-50 PF02786: CPSase_L_D2" amino acids 116 to 322 (207 residues), 239.2 bits, see alignment E=1.6e-74 PF02222: ATP-grasp" amino acids 138 to 292 (155 residues), 37.6 bits, see alignment E=8.2e-13 PF07478: Dala_Dala_lig_C" amino acids 139 to 291 (153 residues), 46.2 bits, see alignment E=1.8e-15 PF02785: Biotin_carb_C" amino acids 336 to 443 (108 residues), 118.7 bits, see alignment E=5.9e-38 PF21139: BT_MCC_alpha" amino acids 460 to 571 (112 residues), 48.1 bits, see alignment E=6.7e-16 PF00364: Biotin_lipoyl" amino acids 594 to 659 (66 residues), 61.2 bits, see alignment E=3.2e-20

Best Hits

KEGG orthology group: K01968, 3-methylcrotonyl-CoA carboxylase alpha subunit [EC: 6.4.1.4] (inferred from 62% identity to psu:Psesu_1647)

Predicted SEED Role

"Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4)" in subsystem HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism or Serine-glyoxylate cycle (EC 6.4.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.4.1.4

Use Curated BLAST to search for 6.4.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (665 amino acids)

>LRK53_RS07685 acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha (Rhodanobacter sp000427505 FW510-R12)
MFERVLIANRGEIACRVIRTCRRLGIRTIAVYSEADKDAQHVRLADEAWPIGGPLPTESY
LRIDAILDAAKNSGAQAIHPGYGFLSENTAFSRACRDAGIVFIGPDPESIEAMGSKAAAK
HLMAKHAVPLVPGYDGDEQDNAYLAEQAHAIGYPLIIKPSAGGGGKGMQIVRSDAEFAEA
LATAQRVALAAFGDASMLLERYVEQPRHIEFQVFGDRHGHVIHLNERECSAQRRYQKVLE
ETPSPFLDDTRRQAMGAAAVAAAKAVDYVGAGTVEFIVAQDGTFFFMEMNTRLQVEHPVT
ELTLGLDLVEWQLRVANGEALPLTQDEVASHGHAIEVRIYAEDPDQNFLPGSGKLHTLRL
PAPSAHVRLDGGVVEGDTVTIFYDPMIAKLIVFDADRPQALQRLREALAACEITGPKSNI
GFLERLARHPAVVEGRIDTGYLDRHLDEFLVGDAAPTAGVLFAAATAVLLRDESHLATAA
SDPHSPWASADAWRISHAGKRIVALTWREQRFEIEARGHAGDYQLRHGETGCSVRGARLL
DDHLSARFDGESRRVPLRADAAHVLLHDAHGQRYSFARAAAFAWASSDDAGGNQVIAPMP
GRIVLVKAKAGDTVEQGQELLVMEAMKMELALKAPRAGTIESVNATQGEFVEADAVLVRF
ADRAS