Protein Info for LRK53_RS07170 in Rhodanobacter sp000427505 FW510-R12

Annotation: leucine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 940 TIGR00396: leucine--tRNA ligase" amino acids 17 to 373 (357 residues), 575.1 bits, see alignment E=1.3e-176 PF00133: tRNA-synt_1" amino acids 27 to 237 (211 residues), 102.1 bits, see alignment E=6.4e-33 amino acids 475 to 630 (156 residues), 33.5 bits, see alignment E=4.1e-12 amino acids 677 to 717 (41 residues), 26.2 bits, see alignment (E = 6.9e-10) PF09334: tRNA-synt_1g" amino acids 51 to 183 (133 residues), 63.6 bits, see alignment E=3.1e-21 PF13603: tRNA-synt_1_2" amino acids 233 to 374 (142 residues), 214.5 bits, see alignment E=1.8e-67 PF08264: Anticodon_1" amino acids 778 to 903 (126 residues), 67.2 bits, see alignment E=3.1e-22

Best Hits

Predicted SEED Role

"Leucyl-tRNA synthetase (EC 6.1.1.4)" (EC 6.1.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (940 amino acids)

>LRK53_RS07170 leucine--tRNA ligase (Rhodanobacter sp000427505 FW510-R12)
MQDIQTPTTPDQDEAAYRPQAVEAAAQRYWNAQRAYAVKEDASRPKFYCLSMLPYPSGAL
HMGHVRNYTIGDVISRYQRMQGKNVLQPMGWDAFGLPAENAAIKNATAPAKWTYANIEHM
RSQLKSLGYAIDWSREFATCRPEYYRWEQLMFTRLLKKGLAYRKNAVVNWDPVDHTVLAN
EQVVDGRGWRSGAVVEKREIPQWFLKITDYAQELLDGLDTLPGWPDAVKTMQRNWLGRSE
GLEIHFAVEGEAAPLTVFTTRPDTLMGVTFVSIAGEHPLAHKAAHGNPQLAAFLDELKHG
GVSEAELETQDKRGMATGLYAIHPLSGERVPVWVANFVLMGYGTGAVMAVPGHDERDFAF
AHKYGLPIRQVIALQPSLPPLGEGAPSLIKDGADAGTGGARGNAEPSPPAPLPVGEGSGY
DPDVWHDWYSDKAHPGMVVVNSGAMDGKDYRAAFDYIAGVLEADGKGRRRVNWRLRDWGV
SRQRYWGCPIPVIYCSACGAVPVPEDQLPVVLPEDVAFSGVQSPIKADPEWRKTTCPQCG
GPAERETDTFDTFMESSWYYARYTSPGANAQIDERANYWLPVDQYIGGIEHAIMHLLYFR
FYHKLLRDAGMVHSDEPARNLLCQGMVIAETFYRDHADGSKDWINPAAVEVLRDDKGRVV
GAVLKADGQSVHIGGTEKMSKSKNNGIDPQTMVDRYGADTVRLFSMFAAPPEQSLEWSEA
GVEGMARFLKRLWREVTTHAAGPDHKAVAAAHPASLPLQAGEGAAGVIDPAALDAGQKAL
RRQLHETIQKVSDDFGRRHAFNTAIAALMELLNALGRFNDQSEQGRAVRHEALEAMVLLL
NPVVPHVSHALWQVLGHAETVLEDQPWPQVDAAALVRDTLTLAVQVNGKLRATIEVAASA
SKDEAEALARAQPQVAHFLEGVTVRKVIVVPGKIVNIVAG