Protein Info for LRK53_RS06805 in Rhodanobacter sp000427505 FW510-R12

Annotation: prolyl aminopeptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 319 TIGR01249: prolyl aminopeptidase" amino acids 14 to 317 (304 residues), 497.5 bits, see alignment E=6.7e-154 PF00561: Abhydrolase_1" amino acids 40 to 303 (264 residues), 130.2 bits, see alignment E=2.1e-41 PF12697: Abhydrolase_6" amino acids 42 to 303 (262 residues), 41.2 bits, see alignment E=6.4e-14 PF12146: Hydrolase_4" amino acids 66 to 301 (236 residues), 43.2 bits, see alignment E=6.1e-15 PF08386: Abhydrolase_4" amino acids 260 to 306 (47 residues), 21.2 bits, see alignment 5.1e-08

Best Hits

Swiss-Prot: 72% identical to PIP_XYLFT: Proline iminopeptidase (pip) from Xylella fastidiosa (strain Temecula1 / ATCC 700964)

KEGG orthology group: K01259, proline iminopeptidase [EC: 3.4.11.5] (inferred from 73% identity to xac:XAC0909)

Predicted SEED Role

"Proline iminopeptidase (EC 3.4.11.5)" in subsystem Proline, 4-hydroxyproline uptake and utilization (EC 3.4.11.5)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.11.5

Use Curated BLAST to search for 3.4.11.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (319 amino acids)

>LRK53_RS06805 prolyl aminopeptidase (Rhodanobacter sp000427505 FW510-R12)
MPSSPLRSLYPEIEPFDSGFLPVSPVHTLYYEQSGNPHGKPVVFLHGGPGGGTNPKCRRF
FDPAIYRIVLFDQRGCGQSTPHAELTDNTTWDLVADIERLRGHLGIDRWQVFGGSWGSTL
ALAYAQTHPDKVTELVLRGIFMLRRWELEWFYQKGCDALYPDAWETYLNAIPEVERGDLM
SAYHRRLTSTDAKTRTDAARAWSVWEGATSFLWQDQSHIESSGEDEFALAFARIECHYFV
NGGFFEHDDQLLRNVGRIRHIPAVIVQGRYDVVCPLRSAWDLHRAWSEAELRIVQDAGHS
AFEPGNVHELIEATDRFGR