Protein Info for LRK53_RS05855 in Rhodanobacter sp000427505 FW510-R12

Annotation: isoaspartyl peptidase/L-asparaginase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 331 signal peptide" amino acids 1 to 18 (18 residues), see Phobius details PF01112: Asparaginase_2" amino acids 8 to 310 (303 residues), 385.4 bits, see alignment E=9.4e-120

Best Hits

Swiss-Prot: 49% identical to IAAA_ECOLI: Isoaspartyl peptidase (iaaA) from Escherichia coli (strain K12)

KEGG orthology group: K13051, beta-aspartyl-peptidase (threonine type) [EC: 3.4.19.5] (inferred from 68% identity to gdj:Gdia_3233)

MetaCyc: 49% identical to isoaspartyl dipeptidase proenzyme (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Isoaspartyl aminopeptidase (EC 3.4.19.5) @ Asp-X dipeptidase" (EC 3.4.19.5)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.4.19.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (331 amino acids)

>LRK53_RS05855 isoaspartyl peptidase/L-asparaginase (Rhodanobacter sp000427505 FW510-R12)
MSHAAVSPVLVIHGGAGVVKRDMTPAKEQAVRAAMTRALQDGYAKLKAGGSALDAVAAAI
TVLEDDPNFNAGKGAVFTHEGINELDAAIMDGDTHRAGAVAGVTRIKNPILLARAVMEKT
PYVMLSGQGAEAFAKSIGMPLVDPSYFRTEQRWQQLQQALKEDAAKQPHADVETAKHFGT
VGAVALDAQGRLAAGTSTGGMTDKRWGRVGDSPIIGAGTYANSGCAVSGTGWGEFYIRSV
VAHQICMKVTQMRVPLKRAAAEVVNQEIPSMGGNGGAIALDAEGHISIPFNTDGMYRGWI
AADGVPHVALYGDEDDGTAELPAPADSAAQP