Protein Info for LRK53_RS05600 in Rhodanobacter sp000427505 FW510-R12

Annotation: valine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 987 transmembrane" amino acids 498 to 518 (21 residues), see Phobius details TIGR00422: valine--tRNA ligase" amino acids 1 to 651 (651 residues), 877.3 bits, see alignment E=5.7e-268 PF00133: tRNA-synt_1" amino acids 14 to 625 (612 residues), 437.3 bits, see alignment E=1.4e-134 PF09334: tRNA-synt_1g" amino acids 40 to 86 (47 residues), 24.8 bits, see alignment 2.2e-09 PF13603: tRNA-synt_1_2" amino acids 238 to 294 (57 residues), 30.3 bits, see alignment 7.1e-11 PF08264: Anticodon_1" amino acids 697 to 860 (164 residues), 127.7 bits, see alignment E=8.5e-41 PF10458: Val_tRNA-synt_C" amino acids 922 to 986 (65 residues), 58.2 bits, see alignment 2e-19

Best Hits

Predicted SEED Role

"Valyl-tRNA synthetase (EC 6.1.1.9)" (EC 6.1.1.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (987 amino acids)

>LRK53_RS05600 valine--tRNA ligase (Rhodanobacter sp000427505 FW510-R12)
MDKSFEPAQIESTWYARWEASGAFRPSGTGEPYCILLPPPNVTGTLHMGHAFQQTVMDML
VRYHRMRGFNTLWQVGTDHAGIATQKIVENQLAVEEKTRHDLGRDAFVERVWQWKEESGS
TITHQMRRIGAAADWSRERFTMDEGLSAAVRKVFIDWYRAGLIYRGNRLVNWDPLLGTAV
SDLEVNNVERDGHMWSIRYPVVGSDESVVVATTRPETMLGDVAVAVHPEDARYAHLVGRT
LKLPLTDREIPVIADDYVDREFGTGCVKITPAHDFNDYAIGQRHQLPPITIFTLDAKVND
NAPEKYRGLDRYDARKAVLADLEALGLLVETKPHKLQVPVSQRSDAVIEPMLTDQWFVDL
TSDVQQDGRPGGRKAITEPALDAVRNGDIKFVPENWSTTYTQWLDNIQDWCISRQLWWGH
RIPAWYDEAGNIFVGEDEADARAHADTAPVGALRQDEDVLDTWFSSALWPFSTMGWPADG
TVKNERGEVVANWANDQIFLPSAVLVTGFDIIFFWVARMVMATRYFTGRIPFREVYINAI
VRDAEGQKMSKSKGNTLDPLDLIDGIALEPLVEKSTKSLLIPQVRTKVEKRIRKDYPGGI
PAIGTDALRFTFAALASYSRTINFDIKRAEGYKAFCNKLWNAARFVLMNVSPLPAGEGGA
DAPGEGTGGARPSGITAPSPQPLSRGERGSAPVTEAERWILTRLAQTLTEAEEHFRTYRF
DHLAQALYEFVWNEYCDWFLELSKPALNGEDADAAASTRHTLLVVLEAVLRALHPVIPFL
TEEVWQEVAKVFVRSSMSAKNSPASLPDSSTKSILQSTYPRGADFAHDDAATAEIEWFKA
VLSGIRRIRSEMNIAPGKTIPLLLADGDSGDHARVAKFAAQIAFLARTEAPQWIEAGSAE
PAAAAAVVGTLRVLIPLAGLIDLDAEKARLAREIGRIEVEIKKCEGKLGNASFVANAPAE
VVAQERQRIADWNATLGALREQAQKLV