Protein Info for LRK53_RS04995 in Rhodanobacter sp000427505 FW510-R12

Annotation: glycoside hydrolase family 65 protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 752 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details PF03636: Glyco_hydro_65N" amino acids 106 to 299 (194 residues), 63 bits, see alignment E=3.6e-21 PF03632: Glyco_hydro_65m" amino acids 358 to 581 (224 residues), 152.4 bits, see alignment E=1.7e-48

Best Hits

Predicted SEED Role

"Maltose phosphorylase (EC 2.4.1.8) / Trehalose phosphorylase (EC 2.4.1.64)" in subsystem Maltose and Maltodextrin Utilization or Trehalose Biosynthesis or Trehalose Uptake and Utilization (EC 2.4.1.64, EC 2.4.1.8)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.64, 2.4.1.8

Use Curated BLAST to search for 2.4.1.64 or 2.4.1.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (752 amino acids)

>LRK53_RS04995 glycoside hydrolase family 65 protein (Rhodanobacter sp000427505 FW510-R12)
MKLRHALLAGLFLIVAAPAQAATDPSFLLTATAKDFASYFPGQLGNGYQSTLTSPRGTEG
NLAYLVAFMDYAKDDISRPAAVPGWSEIDYSTGHSPAGHFWLNRAALDPAVFQDYQQQLN
MREGTLTTRYRYLDHGRATRIEVTSLVSQASPHLAATRLSITPEFDGEVQLSFALNLWAP
HQPRLPLARLSGDEMQEAVAAHKLKLEPVAPATPDRAVLWYHGDTHVTAADGDAADRTLW
LDGRAEQGLSMAEAAAIELPADLQPSDLRVYRSRYRLALNLTAKVRKGQTYTFTRFVALS
RAGWGGDAKADRTLATAARTAGFDRLLDAHRHAWSELWQSDIRIDGDPQAQQVVHSDLYY
LLSNVTADTAWPTGACGMTPGYAGHVFWDSDTWVFPALLLLHPQRAKSLVMFRSRTLPAA
EARAQARGLRGAMFPWEADPQNGSEQTPHMAYVLGEREIHVNADIAIAQWQYYLASGDRQ
WLARHGWPVIRDVADFWASRATWVADRHRYEIHHVTSVLEAYNDVPNDTFTNASARKALE
IAVAAAAVVGARPDPHWSEVAAQLYIPFSVKDGHHLDFDENVPHDIDAWGDTALPALAYP
SLDLPMSAEVRRNDFDYAMTLTGRSQQEPNSMGLSPVSIAAAALGDAQTAGAWFQRNLTT
RVLQPPFNARTETATNNTGYFLTASGGLLQNILYGFTGLRLKRDGLVEAYAPVLPPAWKS
FTLRHVSLRGRYYDITVRRAADGKPELVRHAL