Protein Info for LRK53_RS04585 in Rhodanobacter sp000427505 FW510-R12

Annotation: phosphoenolpyruvate carboxykinase (GTP)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 594 PF17297: PEPCK_N" amino acids 10 to 219 (210 residues), 294.5 bits, see alignment E=4.9e-92 PF00821: PEPCK_GTP" amino acids 223 to 577 (355 residues), 515.7 bits, see alignment E=5.3e-159

Best Hits

Swiss-Prot: 55% identical to PCKG_ANADE: Phosphoenolpyruvate carboxykinase [GTP] (pckG) from Anaeromyxobacter dehalogenans (strain 2CP-C)

KEGG orthology group: K01596, phosphoenolpyruvate carboxykinase (GTP) [EC: 4.1.1.32] (inferred from 60% identity to scl:sce3007)

Predicted SEED Role

"Phosphoenolpyruvate carboxykinase [GTP] (EC 4.1.1.32)" in subsystem Pyruvate metabolism I: anaplerotic reactions, PEP (EC 4.1.1.32)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.1.32

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (594 amino acids)

>LRK53_RS04585 phosphoenolpyruvate carboxykinase (GTP) (Rhodanobacter sp000427505 FW510-R12)
MASTLEALDRWVAEVAALTRPDRIHWCDGSEAEYQTLVQQMLADGTLIELNQQTHPGCYL
HRSHPQDVARVEHLTLVCHPNREDAGPNNHWMAPAEAHAKIDALFDGCMAGRTMYVIPYC
MGPIDSPLARCGVEITDSPYVVANMKIMTRMGADAQARIEREGSFVKGLHSIGELDPEQR
WIMHFPAELSIKSYGSGYGGNALLGKKCHALRIASNQARSEGWLAEHMLIVGIENPRGET
HYIAAAFPSACGKTNLSMLIPPEGYRRDGWKVWTIGDDICWMHPGPDGRLYAINPEAGFF
GVAPGTSDVTNHNAMRSISHDTIFTNVAVTADNQPWWEGLPGTPVTDWQGRPYDPANGPA
AHPNSRFTVSAKQCPTWSAQAEAPQGVPISAIVFGGRRPSLLPLVMEARDWSHGVLMGAA
MGSETTAAATGAVGVLRRDSMAMKPFCGYHYGDYFAHWLSFDRPGAKLPKIFHVNWFRKG
ADGKFLWPGFGDNLRVLQWMIDRAEGRVQGVETPIGILPGEGELQLDGLKLERSRLDELL
DVDNAGWQAELSAIDDYLASFAPRLPERLRREQQRVAQALESDAGPSRREAAAS