Protein Info for LRK53_RS04435 in Rhodanobacter sp000427505 FW510-R12

Annotation: electron transfer flavoprotein subunit alpha/FixB family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 314 PF01012: ETF" amino acids 4 to 173 (170 residues), 123.9 bits, see alignment E=7e-40 PF00766: ETF_alpha" amino acids 194 to 274 (81 residues), 116.5 bits, see alignment E=4.3e-38

Best Hits

Swiss-Prot: 52% identical to ETFA_BRADU: Electron transfer flavoprotein subunit alpha (etfA) from Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)

KEGG orthology group: K03522, electron transfer flavoprotein alpha subunit (inferred from 75% identity to sml:Smlt0645)

Predicted SEED Role

"Electron transfer flavoprotein, alpha subunit" in subsystem Acetyl-CoA fermentation to Butyrate

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (314 amino acids)

>LRK53_RS04435 electron transfer flavoprotein subunit alpha/FixB family protein (Rhodanobacter sp000427505 FW510-R12)
MSKILVIAEHLGGKLNSSTARAVSAAVAVKGEAIDVLVLADNVDVIAAEAAKIDGVSRVL
TVAKPENAHPLAAVLAPQIAKAAAGYSHVFVPSTTFGKDVAPRVAALLGVAQVSDVMSVE
ATHTFKRPIYAGNAIITVEADAAHTVVATIRSASWPAATTGANSAPVEAMAIDAALPTHT
RFVELKQGASDRPDLQSASKVVSGGRGVGSKENFDIIFKFADKIGAAVGASRAAVDAGYV
PSDLQVGQTGKIIAPDLYMAIGISGAIQHLTGIKDAGTIVAINKDGEAPIFEVADFGLVG
DLFQILPALGEALR