Protein Info for LRK53_RS04425 in Rhodanobacter sp000427505 FW510-R12

Annotation: SDR family oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF04321: RmlD_sub_bind
  • amino acids 1 to 238 (238 residues), 92.1 bits, E=5.2e-30
• PF01370: Epimerase
  • amino acids 4 to 150 (147 residues), 58.5 bits, E=1.1e-19
• PF16363: GDP_Man_Dehyd
  • amino acids 4 to 150 (147 residues), 30.8 bits, E=3.2e-11

Best Hits

KEGG orthology group: K00067, dTDP-4-dehydrorhamnose reductase [EC: 1.1.1.133] (inferred from 60% identity to rfe:RF_0538)

MetaCyc: 48% identical to UDP-N-acetyl-beta-L-rhamnosamine dehydrogenase (Vibrio cholerae O37)
RXN-14759 [EC: 1.1.1.367]; 1.1.1.- [EC: 1.1.1.367]

Predicted SEED Role

"dTDP-4-dehydrorhamnose reductase (EC 1.1.1.133)" in subsystem Rhamnose containing glycans or dTDP-rhamnose synthesis or linker unit-arabinogalactan synthesis (EC 1.1.1.133)

MetaCyc Pathways

• O-antigen building blocks biosynthesis (E. coli) (10/11 steps found)
• dTDP-β-L-rhamnose biosynthesis (5/5 steps found)
• UDP-N-acetyl-β-L-quinovosamine biosynthesis (4/4 steps found)
• UDP-N-acetyl-β-L-fucosamine biosynthesis (2/4 steps found)
• superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (11/24 steps found)
• superpathway of dTDP-glucose-derived O-antigen building blocks biosynthesis (7/19 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (13/33 steps found)

KEGG Metabolic Maps

• Polyketide sugar unit biosynthesis
• Streptomycin biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.133

Use Curated BLAST to search for 1.1.1.133 or 1.1.1.367

Protein Sequence (284 amino acids)

>LRK53_RS04425 SDR family oxidoreductase (Rhodanobacter sp000427505 FW510-R12)
MRLLIVGITGMLGNTLFRYFNQRSGVEVFGTVRDGRAKSRFDLAYHDNIIGGVDADHFDS
VTSAFAQAKPDTVINAVGLIKQLQSSKDPLSALPLNALFPHRLARLCAVAGARLIHISTD
CVFTGAIGNYVEKDIPDAIDLYGRSKLLGEVDYPHAITLRTSIIGHELDSANGLIGWFLS
QQETIRGYTRAIFSGFPTVELARIIERHVLPRPECHGLYHVASSPISKYDLLQLTAAAYD
KDIEMIPDDTLVIDRSLNADRFNKATGYQPPSWPELVRTMKNFG