Protein Info for LRK53_RS04190 in Rhodanobacter sp000427505 FW510-R12

Annotation: dihydrolipoyl dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00364: Biotin_lipoyl
  • amino acids 6 to 77 (72 residues), 74 bits, E=2.2e-24
• PF07992: Pyr_redox_2
  • amino acids 140 to 460 (321 residues), 210.4 bits, E=1.2e-65
• TIGR01350: dihydrolipoyl dehydrogenase
  • amino acids 140 to 591 (452 residues), 495.4 bits, E=7.4e-153
• PF12831: FAD_oxidored
  • amino acids 141 to 179 (39 residues), 32.5 bits, 1.8e-11
• PF01134: GIDA
  • amino acids 141 to 202 (62 residues), 22.2 bits, E=2.1e-08
• PF00070: Pyr_redox
  • amino acids 310 to 380 (71 residues), 54.1 bits, E=5.5e-18
• PF02852: Pyr_redox_dim
  • amino acids 479 to 587 (109 residues), 123.4 bits, E=1.5e-39

Best Hits

KEGG orthology group: K00382, dihydrolipoamide dehydrogenase [EC: 1.8.1.4] (inferred from 69% identity to xcc:XCC0544)

Predicted SEED Role

"Dihydrolipoamide dehydrogenase of pyruvate dehydrogenase complex (EC 1.8.1.4)" in subsystem Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate or TCA Cycle (EC 1.8.1.4)

MetaCyc Pathways

• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (19/22 steps found)
• 2-oxoglutarate decarboxylation to succinyl-CoA (3/3 steps found)
• 2-oxoisovalerate decarboxylation to isobutanoyl-CoA (3/3 steps found)
• glycine biosynthesis II (3/3 steps found)
• glycine cleavage (3/3 steps found)
• pyruvate decarboxylation to acetyl CoA I (3/3 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Glycine, serine and threonine metabolism
• Glycolysis / Gluconeogenesis
• Pyruvate metabolism
• Valine, leucine and isoleucine degradation

Isozymes

Compare fitness of predicted isozymes for: 1.8.1.4

Use Curated BLAST to search for 1.8.1.4

Protein Sequence (604 amino acids)

>LRK53_RS04190 dihydrolipoyl dehydrogenase (Rhodanobacter sp000427505 FW510-R12)
MANTIEIKVPDIGGHDNVPVIEVLVKAGDTVAKEQSLITLESDKATMEIPSSAAGVVKEL
KLKVGDEVSEGAVIAVLEVAGASLPSPSGRGAGGEGTGEARSSAPTEPSPAPSGHPLPQG
EGKASQAASGTGRKADIECKLVVLGSGPGGYSAAFRAADLGVDTVLVERYGTLGGVCLNV
GCIPSKALLHAAAVIDEAEAMAAHGVTFGAPKIDIDKLRSFKSKVVGQLTGGLAGMAKAR
KVRTVEGSGTFVSPHEMEVQTKDGVKLLRFEHAIIAAGSQSVKLPAFPWDDERVMDSTGA
LELKDVPKNLLVVGGGIIGLEMATVYSALGSAVTVVEFMDQLIPGADADLIKPLAKRLGG
KLKGVHLKTKVVSAKATKKGIEVGYEGDSIPETTLFDRVLVAVGRSPNGNKIGADKAGVA
VTERGFINVDTQMRTNVPHIYAIGDLVGQPMLAHKATHEAHVAAQAVAGQKSHFDARVIP
SVAFTDPEIAWVGVTEREAKEKGLKVGVGKFPWAASGRAIGIDRTEGFTKLIFDEATHSI
VGAGIVGVHAGDLISELALAIEMGSEAADIALTVHPHPTLGESVGMAAEIYEGTITDLYL
PKKK