Protein Info for LRK53_RS04185 in Rhodanobacter sp000427505 FW510-R12

Annotation: dihydrolipoyllysine-residue acetyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01348: dihydrolipoyllysine-residue acetyltransferase
  • amino acids 4 to 471 (468 residues), 603.1 bits, E=2.9e-185
• PF00364: Biotin_lipoyl
  • amino acids 6 to 76 (71 residues), 72 bits, E=5.7e-24
• PF02817: E3_binding
  • amino acids 170 to 204 (35 residues), 54.6 bits, 2e-18
• PF00198: 2-oxoacid_dh
  • amino acids 244 to 471 (228 residues), 258.4 bits, E=1.1e-80

Predicted SEED Role

"Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex (EC 2.3.1.12)" in subsystem Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 2.3.1.12)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (23/26 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (19/22 steps found)
• pyruvate decarboxylation to acetyl CoA I (3/3 steps found)
• pyruvate fermentation to acetate II (3/3 steps found)
• pyruvate fermentation to acetate V (3/3 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (13/17 steps found)
• pyruvate fermentation to acetate and (S)-lactate I (3/4 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (19/27 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (18/26 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (6/10 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Glycolysis / Gluconeogenesis
• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.12

Use Curated BLAST to search for 2.3.1.12

Protein Sequence (471 amino acids)

>LRK53_RS04185 dihydrolipoyllysine-residue acetyltransferase (Rhodanobacter sp000427505 FW510-R12)
MADLKEARVPDIGHADVPVIEVLVKAGDRVEKEQSLITLESDKATMEVPAPFAGTVKEMK
LKVGDEVSEGAVIALIEIDSHKEADGAAVSASPSPAAGEGRGEGAPEAVKPPAAAPAPAP
APAPAPAPAEPKPAPIAGQGVQPGNAPEGDMPPRARPPMDASIVMPGDAPYASPAIRAFA
RELGVDIQQVKGSGRGGRIQREDVSTYVKHALASGARPVAGAAASVGGLNLLPWPKVDFA
KFGEIEEKPLTRIQKISGANLSRNWAMIPHVTQHEDADITEMEAFRKQLGAENKDLKISP
LVFQIKAVVAALKAFPQFNASLDESGEKLILKKFFHIGIAVDTPDGLVVPVIRDCDKKGL
LDLARDLAEISKKAREKKLGPAEMSGGCFSISSLGGIGGTYFTPIVNAPEVAILGVSKSV
TKPVWNGKEFAPRLILPLSLSYDHRVIDGALAARFASFLANQLGDIRRLLL