Protein Info for LRK53_RS04070 in Rhodanobacter sp000427505 FW510-R12

Annotation: NADP-dependent malic enzyme

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00390: malic
  • amino acids 22 to 115 (94 residues), 76.2 bits, E=4.4e-25
  • amino acids 116 to 153 (38 residues), 24.5 bits, 3.3e-09
• PF03949: Malic_M
  • amino acids 167 to 359 (193 residues), 73.1 bits, E=3.6e-24
• PF01515: PTA_PTB
  • amino acids 430 to 750 (321 residues), 310.8 bits, E=1.9e-96

Best Hits

Swiss-Prot: 60% identical to MAO2_ECOLI: NADP-dependent malic enzyme (maeB) from Escherichia coli (strain K12)

KEGG orthology group: K00029, malate dehydrogenase (oxaloacetate-decarboxylating)(NADP+) [EC: 1.1.1.40] (inferred from 72% identity to xca:xccb100_0853)

MetaCyc: 60% identical to malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) (Escherichia coli K-12 substr. MG1655)
RXN-16819 [EC: 4.1.1.101]; Malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+)). [EC: 4.1.1.101, 1.1.1.40]

Predicted SEED Role

"NADP-dependent malic enzyme (EC 1.1.1.40)" in subsystem Pyruvate metabolism I: anaplerotic reactions, PEP (EC 1.1.1.40)

MetaCyc Pathways

• gluconeogenesis I (11/13 steps found)
• L-malate degradation I (1/1 steps found)
• C4 photosynthetic carbon assimilation cycle, NADP-ME type (4/7 steps found)
• C4 photosynthetic carbon assimilation cycle, PEPCK type (8/14 steps found)
• methylgallate degradation (2/6 steps found)
• protocatechuate degradation I (meta-cleavage pathway) (3/8 steps found)
• superpathway of vanillin and vanillate degradation (3/10 steps found)
• syringate degradation (3/12 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of terpenoids and steroids
• Carbon fixation in photosynthetic organisms
• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.40 or 4.1.1.101

Protein Sequence (763 amino acids)

>LRK53_RS04070 NADP-dependent malic enzyme (Rhodanobacter sp000427505 FW510-R12)
MSLPEELRLAALEYHRLPRPGKIKVTPTTSLLTQRDLSLAYSPGVAAACDAIVEDPTTAA
EYTARANLVAVITNGTAVLGLGNIGPLAAKPVMEGKGVLFQKFAGIDVFDIEIAENDPDK
LVDIIASLEPTFGGINLEDIKAPECFIVERKLRERMKIPVFHDDQHGTSIIVGAALLNAL
IVVGKKIGDIRVATTGAGAAGISCLDMLVALGVKPEHILAFDRDGVIHSGRTNLDPDKQR
YARDTKARTLAEVVEGADVFLGLSAGGILKPEMVATMAARPVIFALANPNPEITPEEAKA
VRPDCIMATGRSDYPNQVNNALCFPYLFRGALDVGATVINEPMKIACVKAIAALARRESS
DVSARAYGGKPPSFGAEYLIPQPFDPRLLIQLPPAVARAAMESGVATRPIADFAAYNDQL
TSFVFRTGLLMKPVFERAKSAPARLVYAEGEEETVLRAVQVVVDEGLAKPILIGRPEVIE
KRIKRAGLRIKLGVDVEICNINSDPRFDDYWQHYHRLMQRRGVTPSMAKAVVRSRPTAIA
ALMVERGEADGMICGLVGQYQSKLRYIRDIIGLDAGVVEPSAMAAVATDKGTFFYLDTHV
QDDPCPERIAEATLQASIRLKLFGITPKIALLSGGNFGSRDTCGAKKMNRALELIRARAP
RLEVEGEMQADVALVPSLREKLFPNSRLEGKANVFVFPNLDAANIAYNMTRMLSDGVVIG
PILMGVAKPAHILMPQSTVRRVVNMTAVACVEAQIRAQNRQSG