Protein Info for LRK53_RS03380 in Rhodanobacter sp000427505 FW510-R12

Annotation: malate synthase A

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF20656: MS_N
  • amino acids 20 to 72 (53 residues), 68.6 bits, 4.1e-23
• TIGR01344: malate synthase A
  • amino acids 25 to 530 (506 residues), 728.9 bits, E=1.2e-223
• PF01274: MS_TIM-barrel
  • amino acids 163 to 407 (245 residues), 342.7 bits, E=1.4e-106
• PF20659: MS_C
  • amino acids 413 to 530 (118 residues), 105 bits, E=5.1e-34

Best Hits

Swiss-Prot: 61% identical to MASY_MYXXD: Malate synthase (mls) from Myxococcus xanthus (strain DK 1622)

KEGG orthology group: K01638, malate synthase [EC: 2.3.3.9] (inferred from 66% identity to sml:Smlt0231)

MetaCyc: 58% identical to malate synthase A (Escherichia coli K-12 substr. MG1655)
Malate synthase. [EC: 2.3.3.9]

Predicted SEED Role

"Malate synthase (EC 2.3.3.9)" in subsystem Photorespiration (oxidative C2 cycle) (EC 2.3.3.9)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (23/26 steps found)
• glyoxylate cycle (6/6 steps found)
• superpathway of glyoxylate bypass and TCA (10/12 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• glycolate and glyoxylate degradation II (1/2 steps found)
• chitin deacetylation (2/4 steps found)
• superpathway of glycol metabolism and degradation (3/7 steps found)
• L-arabinose degradation IV (3/8 steps found)
• D-xylose degradation IV (1/7 steps found)
• crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered) (2/14 steps found)
• superpathway of pentose and pentitol degradation (5/42 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.3.9

Protein Sequence (530 amino acids)

>LRK53_RS03380 malate synthase A (Rhodanobacter sp000427505 FW510-R12)
MAVPQERLDLGTVEIHADPARYAGILTPAALDFLARLHRRFEPTRQQLLQARRERQAGYD
AGGLPDFRADTASIRAAEWTVAPIPAALQDRRVEITGPVERKMIINALNSGAKVFMADFE
DSSAPTFANQLDGQLNLLDAVNGTIDFTSPEGKSYRVNDNPAVLVVRPRGWHLHDKFFSV
AGEPMAGALVDFGLFAFHNARALHGSDRGPYFYLPKLEAMEEAALWDAVMAVAEDELQLP
VGTMKATVLIETLPAAFQMHEILHALRRRAVGLNCGRWDYIFSYLKTLRGHRDRLLPERG
QVQMTVPFLKAYSDLLIQTCHRRGAFAMGGMAAQIPIKGDEAANEAALAKVRADKLREVK
AGHDGTWVAHPALVPVAQAVFDEYMPAPNQLGVLREDVRVSREQLLAAPAGTVTRAGFDN
NVEVCLRYTAAWLDGLGCVPIHHLMEDAATAEIARAQLWQWLHHGDLEFPDHAPIDFALF
DHAVAAHTHRLRDSRHPGAARADAAAALLGAMTHADQLGDFLTVPAYEQL