Protein Info for LRK53_RS03120 in Rhodanobacter sp000427505 FW510-R12

Annotation: 2-isopropylmalate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 544 PF00682: HMGL-like" amino acids 21 to 289 (269 residues), 303.1 bits, see alignment E=2.5e-94 TIGR00973: 2-isopropylmalate synthase" amino acids 21 to 514 (494 residues), 637.8 bits, see alignment E=5.2e-196 PF22617: HCS_D2" amino acids 304 to 384 (81 residues), 99.1 bits, see alignment E=2.3e-32 PF08502: LeuA_dimer" amino acids 386 to 516 (131 residues), 96.1 bits, see alignment E=2.5e-31

Best Hits

KEGG orthology group: K01649, 2-isopropylmalate synthase [EC: 2.3.3.13] (inferred from 57% identity to sur:STAUR_3608)

Predicted SEED Role

"2-isopropylmalate synthase (EC 2.3.3.13)" in subsystem Branched-Chain Amino Acid Biosynthesis or Leucine Biosynthesis (EC 2.3.3.13)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.3.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (544 amino acids)

>LRK53_RS03120 2-isopropylmalate synthase (Rhodanobacter sp000427505 FW510-R12)
MMKTPDQQSGPASDDDVVAGRVRIFDTTLRDGEQAPGFSMDRRAKLRMAQQLEVLGVDVL
EAGFPQASPDDFAAVAQIAGALQGTTVCALARCQDGDIDSAARALEAARHSRIHLFLSTS
PLHREHKLGMSKARVLETAARAIERARGLCHEVEFSAEDALRTEPEFLAEVFSAAVAAGA
STLNAPDTVGYTTPTEIVELFEYLRRHVVGAERVVFSSHCHDDLGMAVANSLAAVSAGAR
QVECTINGIGERAGNAALEEVVMALKVRAPHFGVDTRIDTRQLYPASRLLAELTGQAVPR
NKAIVGANAFAHESGIHQHGMLKHRGTYEIMRPQDVGIAETKLVLGKHSGRAALRQRLQA
LGHTPDDTALDDIFGRFKTLADHGRELHDEDLEALALGHDPHAGGPWQLVQLHASSHLGG
SASASVKLAHDDGREVGEAAIGDGPVDAVLRAIERATGTPLALTRFQIDALGEGADAQGH
AQLSARHAARDWRGHGSSTDIVEAAALAALVIVNRIERQSTLHPQTNKRPSMAARPDAHK
ETTA