Protein Info for LRK53_RS01470 in Rhodanobacter sp000427505 FW510-R12

Annotation: class I SAM-dependent rRNA methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 390 PF17785: PUA_3" amino acids 19 to 68 (50 residues), 52.1 bits, see alignment 1.8e-17 PF10672: Methyltrans_SAM" amino acids 177 to 349 (173 residues), 56.7 bits, see alignment E=9.2e-19 PF01728: FtsJ" amino acids 210 to 321 (112 residues), 29.8 bits, see alignment E=2.2e-10 PF03602: Cons_hypoth95" amino acids 216 to 316 (101 residues), 26 bits, see alignment E=2.7e-09 PF06325: PrmA" amino acids 217 to 271 (55 residues), 30.1 bits, see alignment 1.4e-10 PF13847: Methyltransf_31" amino acids 217 to 335 (119 residues), 36.8 bits, see alignment E=1.4e-12 PF05175: MTS" amino acids 217 to 296 (80 residues), 28 bits, see alignment E=6.1e-10 PF13649: Methyltransf_25" amino acids 221 to 294 (74 residues), 37.8 bits, see alignment E=1e-12

Best Hits

Swiss-Prot: 34% identical to RLMI_ENT38: Ribosomal RNA large subunit methyltransferase I (rlmI) from Enterobacter sp. (strain 638)

KEGG orthology group: K06969, ribosomal RNA large subunit methyltransferase I [EC: 2.1.1.-] (inferred from 59% identity to psu:Psesu_3063)

Predicted SEED Role

"LSU m5C1962 methyltransferase RlmI" in subsystem Ribosome biogenesis bacterial

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.-

Use Curated BLAST to search for 2.1.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (390 amino acids)

>LRK53_RS01470 class I SAM-dependent rRNA methyltransferase (Rhodanobacter sp000427505 FW510-R12)
MNTPAALPVIRLKSDRAPGHPWVWSAQLHKPEAELPPGSVVEVHDAKGRFVGRALWNGHA
RIALRLLDTHADAVIDADWIAARIARAVQLRRDWLQLDAVSDAWRVVHSEGDGLSGLIVD
RYADVLVIEYFAAGMWRFREAIHTALLTHFPGAQLYWFAESHVQKQESFDCRSPEVPAPF
EVHEHGLRFHAAPGLGHKTGFFADQRDNRKRFAELAKDRRVLDLCCNAGGFAVHAMAAGA
REAVGVDLDASILEVARANAAANNVAATFEQADIFDWVRAAVTRGERYDAVVLDPAKLTR
DRSKVMDALKKYFAMNRMVLDIIPPGGLLLTCSCTGLVGEAEFLEMLRRVALNAGREIQV
LEVRGPGADHPFRTDVPEGRYLKAAFCRVE