Protein Info for LRK53_RS01130 in Rhodanobacter sp000427505 FW510-R12

Annotation: multifunctional CCA addition/repair protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 415 PF01743: PolyA_pol" amino acids 3 to 122 (120 residues), 64.8 bits, see alignment E=1e-21 PF12627: PolyA_pol_RNAbd" amino acids 149 to 212 (64 residues), 46.9 bits, see alignment E=1.9e-16

Best Hits

Swiss-Prot: 66% identical to CCA_STRMK: Multifunctional CCA protein (cca) from Stenotrophomonas maltophilia (strain K279a)

KEGG orthology group: K00974, tRNA nucleotidyltransferase (CCA-adding enzyme) [EC: 2.7.7.72 3.1.3.- 3.1.4.-] (inferred from 66% identity to sml:Smlt4008)

MetaCyc: 55% identical to fused CCA tRNA nucleotidyltransferase/phosphohydrolase (Escherichia coli K-12 substr. MG1655)
TRNA-CYTIDYLYLTRANSFERASE-RXN [EC: 2.7.7.72]

Predicted SEED Role

"tRNA nucleotidyltransferase (EC 2.7.7.21) (EC 2.7.7.25)" in subsystem Polyadenylation bacterial or tRNA nucleotidyltransferase (EC 2.7.7.21, EC 2.7.7.25)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.-, 3.1.4.-

Use Curated BLAST to search for 2.7.7.21 or 2.7.7.25 or 2.7.7.72 or 3.1.3.- or 3.1.4.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (415 amino acids)

>LRK53_RS01130 multifunctional CCA addition/repair protein (Rhodanobacter sp000427505 FW510-R12)
MQIFLVGGAVRDKLLKRPVVDRDHVVVGATPEQLLALGYKPVGKDFPVFLHPATSEEYAL
ARTERKSGRGYHGFVFQADPTITLEQDLARRDLTINAIAEDEHGVLTDPFGGVRDLEARL
LRHASPAFVEDPVRVLRVARFAARFAPLGFTVAAETMALMRQMVADGEVDHLVPERVWAE
TRKALGEPQPSMFLRVLRECGALAVLFPEVDALYGVPQRAEFHPEIDTGVHMELVLDAAA
RLAPGNDVVGFCALTHDLGKALTPAAELPRHIGHEHRGVAPLRALAARLKVPTEHATLAE
LVCREHLNVHRALELKPTTVLKLLGALDALRRPARLELFLAACEADQRGRRGHEYDAYPQ
ADYLRAARTAAAAVESADFVARGLAGPAIGQAMEAARIHAIAALRTQPSPSGSAV