Protein Info for LRK53_RS01065 in Rhodanobacter sp000427505 FW510-R12

Annotation: type I restriction-modification system subunit M

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 485 PF12161: HsdM_N" amino acids 5 to 130 (126 residues), 42.9 bits, see alignment E=1.5e-14 PF02384: N6_Mtase" amino acids 141 to 448 (308 residues), 244.3 bits, see alignment E=3.9e-76 PF01170: UPF0020" amino acids 172 to 281 (110 residues), 26.4 bits, see alignment E=1e-09 PF07669: Eco57I" amino acids 256 to 356 (101 residues), 33 bits, see alignment E=1.3e-11

Best Hits

Swiss-Prot: 59% identical to T1ME_ECOLX: Type I restriction enzyme EcoEI M protein (hsdM) from Escherichia coli

KEGG orthology group: K03427, type I restriction enzyme M protein [EC: 2.1.1.72] (inferred from 78% identity to pzu:PHZ_c0983)

Predicted SEED Role

"Type I restriction-modification system, DNA-methyltransferase subunit M (EC 2.1.1.72)" in subsystem Restriction-Modification System (EC 2.1.1.72)

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.72

Use Curated BLAST to search for 2.1.1.72

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (485 amino acids)

>LRK53_RS01065 type I restriction-modification system subunit M (Rhodanobacter sp000427505 FW510-R12)
MSPRSTVKSIQDLMRQDVGVDGDAQRLSQLCWMFFLKIIDDQDQQLELMQDGFTSPIPPR
LQWRNWAADPEGDTGDTLMAFVNDDLIPALKELPALGKQANRARVIRSLFGDAYNYMKSG
HLMRQVINKINTVDFNDLAERKHFGEVYEQLLNDLQNAGNAGEYYTPRAVTAFMVDRIDP
HPGEKLFDPACGTGGFLTCAIRHMRDHYVKSVADEEALQAGLRAVEKKPLPHMLCVTNML
LHGIEDPSFVQHDNTLARPYISWEQKERVDIILANPPFGGKEEDGIESNFPAHFRTKETA
DLFLALFIRLLKPGGRAAIVLPDGSLFGEGVKTRLKEHLLEECSLHTIVRLPNSVFKPYA
SIGTNLLFFEKGSPTQDIWYYEHRVPEGQKAYSMTRPIKIEHLQDCVDWWGGACREGRVE
TPQAWKVTADEVKARGYNLDIKNPHTEAEDHGDPKALLAELDAAEAETARLRDQLKAILA
EALAR