Protein Info for LRK53_RS00665 in Rhodanobacter sp000427505 FW510-R12

Annotation: phosphogluconate dehydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 605 TIGR01196: phosphogluconate dehydratase" amino acids 5 to 604 (600 residues), 980.8 bits, see alignment E=1.1e-299 PF00920: ILVD_EDD_N" amino acids 70 to 381 (312 residues), 319 bits, see alignment E=3.3e-99 PF24877: ILV_EDD_C" amino acids 410 to 602 (193 residues), 179.7 bits, see alignment E=4.2e-57

Best Hits

Swiss-Prot: 63% identical to EDD_RHIME: Phosphogluconate dehydratase (edd) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K01690, phosphogluconate dehydratase [EC: 4.2.1.12] (inferred from 66% identity to azc:AZC_2424)

MetaCyc: 60% identical to phosphogluconate dehydratase (Escherichia coli K-12 substr. MG1655)
Phosphogluconate dehydratase. [EC: 4.2.1.12]

Predicted SEED Role

"Phosphogluconate dehydratase (EC 4.2.1.12)" in subsystem Entner-Doudoroff Pathway (EC 4.2.1.12)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (605 amino acids)

>LRK53_RS00665 phosphogluconate dehydratase (Rhodanobacter sp000427505 FW510-R12)
MTTLHPVVAEVTERLRERSRETRAAYLARIDAADRSGPRRGHLSCGNLAHGFAACGTEDK
AALRSGQHANLAIVTAYNDMLSAHQPYERYPALIRQIARDAGFTAQVAGGVPAMCDGITQ
GRAGMQLSLFSRDVIALATAVALSHDMFDGALFLGICDKIVPGLLIAALSFGHLPAAFVP
SGPMPSGIPNEQKSKVRQAFADGKASKAELLEAEAASYHAPGTCTFYGTANSNQMLMEIM
GLHLPGASFTPPDTPLRDALTAEVVRRVADLSALGDHYLPLGHLIDERAIINGVIGLHAT
GGSTNHLLHLVAIAHAAGIQLRWDDFDALSGVIPLLARVYPNGYADVNQFHDAGGMAFLI
DQLLGAGLLHADVQTIFGTGLEGYTRVPQLDEAGDIAWLPVAKQSGNRGVLRGVDEPFRA
DGGLRMLDGNLGRAVIKVSSVPDDRLLIEAPAIVFNDQDEVAGAFKRGELNRDFVAVVRF
QGPRANGMPELHKLTPTLALLQDRGHRIALLTDGRMSGASGRVPAAIHVTPEAVAGGNLA
KIRDGDLIRLDAANGRLDVLMEAHELDARLPHVADLSGEHSGMGRELFGLFRQAAANADL
GAGVL