Protein Info for IAI46_21605 in Serratia liquefaciens MT49

Annotation: M20 family metallo-hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 438 TIGR01891: amidohydrolase" amino acids 18 to 420 (403 residues), 367.5 bits, see alignment E=3.9e-114 PF01546: Peptidase_M20" amino acids 114 to 429 (316 residues), 120.5 bits, see alignment E=9.3e-39 PF07687: M20_dimer" amino acids 233 to 315 (83 residues), 39.7 bits, see alignment E=4.2e-14

Best Hits

Swiss-Prot: 66% identical to ABGA_ECOLI: p-aminobenzoyl-glutamate hydrolase subunit A (abgA) from Escherichia coli (strain K12)

KEGG orthology group: K12940, aminobenzoyl-glutamate utilization protein A (inferred from 97% identity to spe:Spro_4200)

MetaCyc: 66% identical to p-aminobenzoyl-glutamate hydrolase subunit A (Escherichia coli K-12 substr. MG1655)
3.5.1.-

Predicted SEED Role

"Catalyzes the cleavage of p-aminobenzoyl-glutamate to p-aminobenzoate and glutamate, subunit A" in subsystem p-Aminobenzoyl-Glutamate Utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (438 amino acids)

>IAI46_21605 M20 family metallo-hydrolase (Serratia liquefaciens MT49)
MNNPLSMLVTDLYPQLQAWRRDFHRYAESGWFEFRTATLVAEELDRLGYRLQLGREVINA
DARMGLPSAEALQAQEQRAREQGALEKWLPHFSGGFAGIVATLDTGRPGPVIAYRVDMDA
LDLNEKLQPDHLPFREGFASCNSGMMHACGHDGHTTIGLGLAHVFKSLEPQLNGTIKLIF
QPAEEGTRGAKSMVEAGVVDDVDFFTAVHIGTGVPAGELVCGSDSFMATSKLDVTYRGVA
AHAGAKPEDGRNALLAAAQATLGLYAIPRHSEGSSRINVGVLQAGTGRNVIADHAFMKVE
TRGATNAINEFVYQQALNVIEGAAKIHGVECDIALMGAAQSSKPTQPWVDYIHRQAQGIA
ELSSVVDRREQAAGSEDATYMMERVKSHGGQASYVIFGTELSAGHHNEKFDFNEQVMAVA
VKTLASLALNLPTFGSQA