Protein Info for IAI46_04840 in Serratia liquefaciens MT49

Annotation: tRNA guanosine(34) transglycosylase Tgt

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 374 TIGR00430: tRNA-guanine transglycosylase" amino acids 3 to 367 (365 residues), 626.7 bits, see alignment E=1.2e-192 TIGR00449: tRNA-guanine family transglycosylase" amino acids 3 to 367 (365 residues), 590.1 bits, see alignment E=1.5e-181 PF01702: TGT" amino acids 10 to 364 (355 residues), 563.9 bits, see alignment E=7.2e-174

Best Hits

Swiss-Prot: 99% identical to TGT_SERP5: Queuine tRNA-ribosyltransferase (tgt) from Serratia proteamaculans (strain 568)

KEGG orthology group: K00773, queuine tRNA-ribosyltransferase [EC: 2.4.2.29] (inferred from 99% identity to spe:Spro_1061)

MetaCyc: 90% identical to tRNA-guanine transglycosylase (Escherichia coli K-12 substr. MG1655)
tRNA-guanine transglycosylase. [EC: 2.4.2.29]

Predicted SEED Role

"tRNA-guanine transglycosylase (EC 2.4.2.29)" in subsystem Queuosine-Archaeosine Biosynthesis (EC 2.4.2.29)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.2.29

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (374 amino acids)

>IAI46_04840 tRNA guanosine(34) transglycosylase Tgt (Serratia liquefaciens MT49)
MKYELDTTDGRARRGRLIFDRGVVETPAFMPVGTYGTVKGMTPEEVKETGAQILLGNTFH
LWLRPGQEIMKLHGDLHDFMQWHGPILTDSGGFQVFSLGAMRKIKEEGVHFRNPINGDKV
FLSPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSMEMSLRWAQRSRQRFDELNNKN
ALFGIIQGGVYEDLRDVSVKGLVDIGFDGYAVGGLAVGEPKEDMHRILEHVCPQIPEDKP
RYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTDGVVKIRNAKHKDDTSPLDK
DCDCYTCRNYSRAYLHHLDRCNEILGARLNTIHNLRHYQRLMAGLREAIEQGKLELFVAD
FYGRIGKPIPPLNA