Protein Info for IAI46_02400 in Serratia liquefaciens MT49

Annotation: N-6 DNA methylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 540 PF12161: HsdM_N" amino acids 6 to 129 (124 residues), 44.9 bits, see alignment E=3.7e-15 PF02384: N6_Mtase" amino acids 138 to 509 (372 residues), 194.8 bits, see alignment E=4.8e-61 PF01170: UPF0020" amino acids 179 to 313 (135 residues), 23.6 bits, see alignment E=7.5e-09 PF07669: Eco57I" amino acids 281 to 385 (105 residues), 39.7 bits, see alignment E=1.1e-13

Best Hits

KEGG orthology group: K03427, type I restriction enzyme M protein [EC: 2.1.1.72] (inferred from 86% identity to pay:PAU_03937)

Predicted SEED Role

"Type I restriction-modification system, DNA-methyltransferase subunit M (EC 2.1.1.72)" in subsystem Restriction-Modification System (EC 2.1.1.72)

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.72

Use Curated BLAST to search for 2.1.1.72

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (540 amino acids)

>IAI46_02400 N-6 DNA methylase (Serratia liquefaciens MT49)
MITRELKSSIDNLWEDFWVGGITNPLTVIEQITYLMYSRMLDSLELKDEKRKQLTNIDFK
PRFAPEQQEFRFSQYSNLGADEMMEVVRDGVFQHFRTLGSNDSLLGNFMKDARLEIVKPS
LLTKAVEMIKNLPLDRGDTKGDLYEYLLSKLTTAGINGQFRTPRHIIRAVVEMMEPNPAR
NEAVCDPACGTGGFLATSYEYLLEKYSSLESIHSELGVNERGELEEQKIFTGDLLTDYRA
HVDQNMFHGYDFDTTMLRIAAMNLIMHGVEQPDIHYQDTMSQSFNDNFPQASKNAFNLIL
ANPPFTGSLDEEDIDPSLLATVKTKKTELLFLARILQMLKVGGRSATIIPQGVLFGSSKA
HQSLRKTLVEDNQLEAVINLPSGVFKPYAGVATAILIFTKGGQTDNVWFYDLHNDGYSLD
DKRTPVKENDLPHLIASWKHYRLNKGLPTDNFIGDKFRSLLEQQYPQGINITSNYQDRTQ
SAFIVSKVDISAEKYDLSINRYKKVIYENEQHEDPKLILNRLKSLEKEILIDLDELEKML