Protein Info for IAI46_01610 in Serratia liquefaciens MT49

Annotation: N-acetylmuramoyl-L-alanine amidase AmiB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 598 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details PF11741: AMIN" amino acids 33 to 116 (84 residues), 25.4 bits, see alignment E=1.9e-09 PF01520: Amidase_3" amino acids 215 to 433 (219 residues), 161 bits, see alignment E=4.4e-51 PF01476: LysM" amino acids 488 to 530 (43 residues), 49.3 bits, see alignment 5.6e-17 amino acids 554 to 596 (43 residues), 46.7 bits, see alignment 3.7e-16

Best Hits

KEGG orthology group: K01448, N-acetylmuramoyl-L-alanine amidase [EC: 3.5.1.28] (inferred from 95% identity to spe:Spro_0428)

Predicted SEED Role

"N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28)" (EC 3.5.1.28)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.28

Use Curated BLAST to search for 3.5.1.28

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (598 amino acids)

>IAI46_01610 N-acetylmuramoyl-L-alanine amidase AmiB (Serratia liquefaciens MT49)
MTYALRKLLVIALIAVLGPLGAASALAASSLSDIKVTNAQREATVSVSFTGPPDYAFFPL
HGPDRVVLDVNQKGKVGGLPLNFSGQNLVKSIRSSAPKDAQSVRLVFDLTQRAKTRVATR
QNGSVYTVVFTIAAEGSANTTVRKAPTPAPVVVNPPTRKAPVVVSEPEPEPAPVRRAPSG
SNPFTNKPTVVAGTASEVTPRSSRVSAGSGDRVVVAIDAGHGGQDPGAIGPNGLKEKNVT
IAIARRLQALLDADPQFKPVLTRNGDYFISVMGRSDVARKQGANVLVSIHADAAPNRSAN
GASVWVLSNRRANSEMAGWLEQHEKQSELLGGAGDLLANSQADPYLSQAVLDLQFGHSQR
VGYDVAVKVLQQLQSVGSLHKRRPEHASLGVLRSPDIPSLLVETGFISNSTEERLLGSSA
YQEKIARAIHNGLRGYFLAHPLQADPKVENRPLDVAAAVNSSTQDVSQPAPIVSSAGSRR
ISGKTQIHVVKRAETLSGIADSYGTTMAALRDLNKLKKDGVWVGQRLKVPAGKTAATVST
VAKAKPSVKKPSRHKVTRGDTLSSIATRYGVSISDLKRVNKLKSDVAPLDRTLTIPQA