Protein Info for IAI46_00855 in Serratia liquefaciens MT49

Annotation: zinc/cadmium/mercury/lead-transporting ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 771 transmembrane" amino acids 162 to 181 (20 residues), see Phobius details amino acids 187 to 207 (21 residues), see Phobius details amino acids 215 to 250 (36 residues), see Phobius details amino acids 387 to 408 (22 residues), see Phobius details amino acids 414 to 442 (29 residues), see Phobius details amino acids 722 to 740 (19 residues), see Phobius details amino acids 745 to 767 (23 residues), see Phobius details PF00403: HMA" amino acids 86 to 141 (56 residues), 44.1 bits, see alignment 4.4e-15 TIGR01512: cadmium-translocating P-type ATPase" amino acids 217 to 556 (340 residues), 324.6 bits, see alignment E=1.6e-100 TIGR01525: heavy metal translocating P-type ATPase" amino acids 217 to 766 (550 residues), 591.8 bits, see alignment E=2.6e-181 TIGR01494: HAD ATPase, P-type, family IC" amino acids 240 to 742 (503 residues), 266.5 bits, see alignment E=5.2e-83 PF00122: E1-E2_ATPase" amino acids 268 to 447 (180 residues), 159.8 bits, see alignment E=1.1e-50 PF00702: Hydrolase" amino acids 465 to 676 (212 residues), 119.5 bits, see alignment E=5e-38 PF08282: Hydrolase_3" amino acids 657 to 710 (54 residues), 28.7 bits, see alignment 2.2e-10

Best Hits

Swiss-Prot: 67% identical to ZNTA_SHISS: Zinc/cadmium/lead-transporting P-type ATPase (zntA) from Shigella sonnei (strain Ss046)

KEGG orthology group: K01534, Cd2+/Zn2+-exporting ATPase [EC: 3.6.3.3 3.6.3.5] (inferred from 93% identity to spe:Spro_0209)

MetaCyc: 67% identical to Zn2+/Cd2+/Pb2+ exporting P-type ATPase (Escherichia coli K-12 substr. MG1655)
3.6.3.3-RXN [EC: 7.2.2.21]; RXN0-5205 [EC: 7.2.2.21, 7.2.2.12]; 7.2.2.- [EC: 7.2.2.21, 7.2.2.12]

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5 or 7.2.2.12 or 7.2.2.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (771 amino acids)

>IAI46_00855 zinc/cadmium/mercury/lead-transporting ATPase (Serratia liquefaciens MT49)
MHNHENHNHQEHNHAEGGCGCNHSHSKTQHGCSSEKQNAVNPGEHSHAHDHGASCCSADD
HGDPDEESDRLAAAPLSGSERYSWKVTGMDCPSCAKKIENAVTAIPGVVNARVLFATEKL
VVDGQSNLSLRIQDAVQQAGFTLLGTQANQAEPPKPSRLGEFGPLLLLTALMIVSWALSM
VNPELSRIAFIATTLVGLAPVATKAIRLIRSGTPFAIETLMSVAAIGALFIGATAEAAMV
LLLFMVGEMLESYAANRARRGVKALMELVPEDALLLQGTERKRVPVSSLRPGDVIEIAPG
GRLPADAELLNPFASFDESALTGESVPVERQQGQKVAAGSLSVDQAAQMKVISEPGKNAI
DRILQLIEEAEERRAPIERFLDRFSRYYTPAIMLLAIAVILVPPLMYAQPWDVWIYRGLT
LLLIGCPCALVISTPAAITSGLAAATRRGALIKGGAALEQLGQVQTIAFDKTGTLTEGKP
TVTDVLPLNGISEQSLLILAAAVEAGSHHPLAQAIINRAEQYGAPLPPAQNRRALAGVGV
EGIVDGKTVLISTPAKLAAGLLNQQGANQVERLENAGKTAVVVLEDGVPIGLLALRDTLR
SDAKQAIAELNALGINGVMLTGDNPRAAAAIAAELGLDYRAGLLPEDKVRAVTELSELRP
TAMIGDGINDAPAMKASSIGIAMGSGTDVALETADAALTHNRLLGVAEMIRISRATHANI
RQNITIALGLKGIFLITTLMGLTGLWLAVLADSGATALVTANALRLLKKRQ