Protein Info for HUW76_04565 in Fusobacterium nucleatum SB010

Annotation: ATP-dependent DNA helicase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 820 PF04851: ResIII" amino acids 140 to 205 (66 residues), 25.1 bits, see alignment E=3.7e-09 PF00270: DEAD" amino acids 151 to 206 (56 residues), 32 bits, see alignment 2.5e-11 PF06733: DEAD_2" amino acids 270 to 376 (107 residues), 31.3 bits, see alignment E=4.1e-11 PF00271: Helicase_C" amino acids 639 to 773 (135 residues), 24.6 bits, see alignment E=6.4e-09 PF13307: Helicase_C_2" amino acids 640 to 803 (164 residues), 174.6 bits, see alignment E=4.8e-55

Best Hits

KEGG orthology group: K03722, ATP-dependent DNA helicase DinG [EC: 3.6.4.12] (inferred from 94% identity to fnu:FN0743)

Predicted SEED Role

"DinG family ATP-dependent helicase YoaA" in subsystem DNA repair, bacterial DinG and relatives

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.12

Use Curated BLAST to search for 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (820 amino acids)

>HUW76_04565 ATP-dependent DNA helicase (Fusobacterium nucleatum SB010)
MDIRDRFSEESLQTIKKYLEEHNNKSMIFKATFDEDEKIQKPFFLSLYKKKSFEETLTKV
GKNEVVIRTTKPNQLYPSDMELELSEELYTRRNIAYCLLSSDLDDFYFVQDIDRIFLEEI
DIENYFAKDGILAKEIKGFEYRQEQEEMAQYIQDAINEDRKIIVEAGTGTGKTLAYLIPS
IKWAVTNKKKVIIATNTINLQEQLLLKDIPLAKSIIKDEFSYVLVKGRNNYVCKRLFNEL
VLGKSIDIETFSMEAREQIEYILKWGNKTKTGDKAELPFEVYPDVWELVQSTTELCLGKK
CPYRKECFYMKTRMEKMEADILISNHHVFFADLNVRAETDFDSEYLILPRYDMVIFDEAH
NVESVARSYFSVEVSKISFTRLLNRIYQKKNKKKKEKSALIRVEDTVDEKNLEDSEQYIY
LLNTLKEEISILQNIGDEYFDEIRKIYETNTEAPIKKSLNNFEMTKSRFLENLREKKDIF
QGKLADFLNLMMSFNNVIDEEKDKNPEVINFNNHLKMFKAYIDSFKFINSFEDDNYIYWL
DINSKRTNVVLTATPLNIAQKLSTVLFDNLDRLVFASATIVVNGNFDYFKKSLGLDEEDC
IEAIIKSPFDYDEQMSVYIPSDIQDSENINAFVSDASKFILNILLKTNGKAFILFTSYTM
LNQIYYSISKKLKDKGFEVFLHGDKPRSQIIKEFKEAENPILFGTTSFWEGVDVQGENLS
NVIITKLPFLVPTDPVVSAISKKIEEDGGNSFMDFQLPEAIIKFKQGVGRLIRKKTDSGN
IFILDNRILKKRYGSLFINALPSQKNIKILEKDDIIEEIE