Protein Info for HUW76_00050 in Fusobacterium nucleatum SB010

Annotation: phenylalanine--tRNA ligase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 338 PF02912: Phe_tRNA-synt_N" amino acids 20 to 88 (69 residues), 87.9 bits, see alignment E=3.4e-29 TIGR00468: phenylalanine--tRNA ligase, alpha subunit" amino acids 40 to 338 (299 residues), 341.9 bits, see alignment E=1.9e-106 PF01409: tRNA-synt_2d" amino acids 93 to 337 (245 residues), 327.6 bits, see alignment E=5.1e-102

Best Hits

Swiss-Prot: 98% identical to SYFA_FUSNN: Phenylalanine--tRNA ligase alpha subunit (pheS) from Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131)

KEGG orthology group: K01889, phenylalanyl-tRNA synthetase alpha chain [EC: 6.1.1.20] (inferred from 98% identity to fnu:FN2123)

Predicted SEED Role

"Phenylalanyl-tRNA synthetase alpha chain (EC 6.1.1.20)" (EC 6.1.1.20)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.20

Use Curated BLAST to search for 6.1.1.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (338 amino acids)

>HUW76_00050 phenylalanine--tRNA ligase subunit alpha (Fusobacterium nucleatum SB010)
MKEEILKVKEEIQNYIKESKTLQRLEEIRVNYMGKKGIFTELSKKMKDLSAEERPKIGQI
INEVKEKINSLLDEKNKALKEKELNERLESEIIDISLPGKKYNYGTIHPINETMELMKNI
FSKMGFDIVDGPEIETVEYNFNALNIPKTHPSRDLTDTFYLNDSIVLRTQTSPVQIRYML
EHGTPFRMICPGKVYRPDYDISHTPMFHQMEGLVVGKDISFADLKGILTHFVKEVFGDRK
VRFRPHFFPFTEPSAEMDVECMICHGKGCRLCKESGWIEIMGCGMVDPEVLKYVGLNPDE
VNGFAFGVGIERVTMLRHGIGDLRAFFENDMRFLKQFK