Protein Info for HMPREF1181_RS14150 in Bacteroides stercoris CC31F

Annotation: 2-isopropylmalate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 496 TIGR00973: 2-isopropylmalate synthase" amino acids 4 to 494 (491 residues), 674.6 bits, see alignment E=3.7e-207 PF00682: HMGL-like" amino acids 6 to 272 (267 residues), 313 bits, see alignment E=1.8e-97 PF08502: LeuA_dimer" amino acids 369 to 495 (127 residues), 123.1 bits, see alignment E=7.6e-40

Best Hits

Swiss-Prot: 84% identical to LEU1_PARD8: 2-isopropylmalate synthase (leuA) from Parabacteroides distasonis (strain ATCC 8503 / DSM 20701 / CIP 104284 / JCM 5825 / NCTC 11152)

KEGG orthology group: K01649, 2-isopropylmalate synthase [EC: 2.3.3.13] (inferred from 95% identity to bhl:Bache_3172)

MetaCyc: 50% identical to 2-isopropylmalate synthase (Escherichia coli K-12 substr. MG1655)
2-isopropylmalate synthase. [EC: 2.3.3.13]

Predicted SEED Role

"2-isopropylmalate synthase (EC 2.3.3.13)" in subsystem Branched-Chain Amino Acid Biosynthesis or Leucine Biosynthesis (EC 2.3.3.13)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.3.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (496 amino acids)

>HMPREF1181_RS14150 2-isopropylmalate synthase (Bacteroides stercoris CC31F)
MSDKLFIFDTTLRDGEQVPGCQLNTVEKIQVARQLEALGVDVIEAGFPISSPGDFNSVIE
ISKAVTWPTICALTRAVQKDIDVAAEALQFAKHKRIHTGIGTSDSHIRYKFNSNREEIIE
RAVAAVKYARRYVEDVEFYAEDAGRTDNEYLARVVEAVIKAGATVVNIPDTTGYCLPGEY
GAKIKYLMEHVDGIHNAVISTHCHNDLGMATANTMAGVLNGARQVEVTINGIGERAGNTS
LEEVAMIIRCHKDIDIETNINTQKIYPTSRMVSSLMNMPVQPNKAIVGRNAFAHSSGIHQ
DGVLKNVQTYEIIDPHDVGIDDNSIVLTARSGRAALKNRLQVLGVSLDQQKLDKIYEDFL
KLADKKKDINDDDILVLAGADRSQNHRIKLEYLQVTSGVGVRSVASIGLNISGEKFEAAA
SGNGPVDAAIKALKKIIDRHMTLKEFTIQAISKGSDDMGKVHMQVEYDNRIYYGFGANTD
IIAASVEAYIDCINKF