Protein Info for HMPREF1181_RS11415 in Bacteroides stercoris CC31F

Annotation: type IA DNA topoisomerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 695 PF01751: Toprim" amino acids 4 to 139 (136 residues), 42.4 bits, see alignment E=1e-14 TIGR01056: DNA topoisomerase III" amino acids 4 to 685 (682 residues), 545.7 bits, see alignment E=9.3e-168 PF01131: Topoisom_bac" amino acids 158 to 577 (420 residues), 406.1 bits, see alignment E=2.9e-125 PF13342: Toprim_Crpt" amino acids 628 to 687 (60 residues), 72.7 bits, see alignment 3e-24

Best Hits

KEGG orthology group: None (inferred from 99% identity to bfr:BF1373)

Predicted SEED Role

"DNA topoisomerase III, Bacteroidales-type (EC 5.99.1.2)" in subsystem DNA topoisomerases, Type I, ATP-independent (EC 5.99.1.2)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.2

Use Curated BLAST to search for 5.99.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (695 amino acids)

>HMPREF1181_RS11415 type IA DNA topoisomerase (Bacteroides stercoris CC31F)
MIALIAEKPSVAKDIARIIGATGRNDGYLSGNGYMVTWAFGHLIQLAMPEAYGVANFRRE
SLPILPPDFQLIPRQVKAEKGYKADPGVLKQLKVIKEVFDQCDRIIVATDAGREGELIFR
YIFHYLNCRKPFVRLWISSLTDKAIREGLDNLQPGERYDNLYFSAKSRSEADWLIGINAT
QALSVAAGQGVFSLGRVQTPTLVMICSRYLENKNFVPAKFWQLKAATASGGINFTAQSTA
KWEQQPEAIAALQRVKDVGQLVVKSVERKEACQEPPLLYDLTTLQKEANTKLNFSADKTL
SIAQSLYEKKVMSYPRTGSRYIPEDVFDEMPERVALLGQYSRFAGYAAGLDGTPLNRRSV
NDGKVTDHHALIITENLPGELSKDERAVYELVAGRMLEAFSGKCVKDVTTALLSGGDTDF
TVKGSVMKEAGWRAVFGEQETGGDEEAASLPPLQEGECLPLSGVDLLEKQTKPKPLHTES
SLLSAMENAGRELEDAELKASLKDAGIGTPATRAAIIETLFARQYIVREKKNLVPTDKGL
AVYRIVRDKKIADVEMTGMWETALAKIEAGSMDADTFRKGIEVYATQITAELLSVQLSVA
TGETCPCPKCGSGRILFYPKVAKCSNVDCTLTIFRNKCDKQLSDKQIVELVTKHKTGLIK
GFKGKNGKAFDASLVLDEQFNVGFSFPEKKAKPKK