Protein Info for HMPREF1181_RS11285 in Bacteroides stercoris CC31F

Annotation: tRNA lysidine(34) synthetase TilS

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 431 TIGR02432: tRNA(Ile)-lysidine synthetase" amino acids 21 to 201 (181 residues), 184.1 bits, see alignment E=2.6e-58 PF01171: ATP_bind_3" amino acids 21 to 197 (177 residues), 170.5 bits, see alignment E=3.3e-54 TIGR02433: tRNA(Ile)-lysidine synthetase, C-terminal domain" amino acids 347 to 377 (31 residues), 25.3 bits, see alignment (E = 8.2e-10)

Best Hits

Swiss-Prot: 64% identical to TILS_BACTN: tRNA(Ile)-lysidine synthase (tilS) from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)

KEGG orthology group: K04075, tRNA(Ile)-lysidine synthase [EC: 6.3.4.-] (inferred from 69% identity to bhl:Bache_1826)

Predicted SEED Role

"tRNA(Ile)-lysidine synthetase (EC 6.3.4.19)" (EC 6.3.4.19)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.3.4.-

Use Curated BLAST to search for 6.3.4.- or 6.3.4.19

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (431 amino acids)

>HMPREF1181_RS11285 tRNA lysidine(34) synthetase TilS (Bacteroides stercoris CC31F)
MEKEKVAQYINRKQLLQPTDKVLVALSGGADSVALLRLLQASGYDCEAAHCNFHLRGAES
DRDEAFVRQLCVKQQVPLHTVHFDTARTAEERHISIEMAARELRYGWFEEIRQKINADAI
AVAHHQDDSVETLLLNLIRGTGINGLRGIRPKNGHIVRPLLCLERKEIISYLNRIGQDYV
TDSTNLQDEYTRNKIRLNLLPMMQEINPSVKESILNTAEHLDGASAIYKQGIDEGIKRVR
TAEGICIERLMQEPAPATLLFEILYPLGFNTAQIRDIYRSLEGQPGKIFTSEHWCIVKDR
EYLLIEAHRPPIQPALAMEEHVFTPDFVIPRDKNTACFDADKLTHPLLLRPWRQGDSFIP
FGMKGRKKVSDYMTDRKFSLLRKEQQWVLCCGEEIIWLIGERTDNRFRIGASTKRVLTVR
LLPAGDSPVTP