Protein Info for HMPREF1181_RS07380 in Bacteroides stercoris CC31F

Annotation: carbamoyl-phosphate synthase (glutamine-hydrolyzing) large subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01369: carbamoyl-phosphate synthase, large subunit
  • amino acids 2 to 1057 (1056 residues), 1438.1 bits, E=0
• PF02786: CPSase_L_D2
  • amino acids 126 to 329 (204 residues), 248.1 bits, E=2.6e-77
  • amino acids 673 to 868 (196 residues), 90.2 bits, E=5.5e-29
• PF02222: ATP-grasp
  • amino acids 137 to 295 (159 residues), 31.5 bits, E=5.4e-11
  • amino acids 681 to 843 (163 residues), 41.7 bits, E=3.8e-14
• PF07478: Dala_Dala_lig_C
  • amino acids 151 to 297 (147 residues), 33.9 bits, E=9.2e-12
  • amino acids 680 to 841 (162 residues), 30.4 bits, E=1.1e-10
• PF02787: CPSase_L_D3
  • amino acids 416 to 495 (80 residues), 102.3 bits, 4.6e-33
• PF02142: MGS
  • amino acids 956 to 1046 (91 residues), 70.2 bits, E=5.4e-23

Best Hits

KEGG orthology group: K01955, carbamoyl-phosphate synthase large subunit [EC: 6.3.5.5] (inferred from 98% identity to bhl:Bache_0155)

Predicted SEED Role

"Carbamoyl-phosphate synthase large chain (EC 6.3.5.5)" in subsystem De Novo Pyrimidine Synthesis or Macromolecular synthesis operon (EC 6.3.5.5)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (38/46 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (9/9 steps found)
• UMP biosynthesis II (6/6 steps found)
• UMP biosynthesis III (6/6 steps found)
• L-arginine biosynthesis I (via L-ornithine) (8/9 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• L-arginine biosynthesis II (acetyl cycle) (8/10 steps found)
• UMP biosynthesis I (5/6 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (7/9 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-glutamate and L-glutamine biosynthesis (5/7 steps found)
• L-citrulline degradation (2/3 steps found)
• L-citrulline biosynthesis (5/8 steps found)
• L-arginine degradation V (arginine deiminase pathway) (2/4 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (12/18 steps found)
• superpathway of L-citrulline metabolism (7/12 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (1/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (1/4 steps found)
• superpathway of arginine and polyamine biosynthesis (10/17 steps found)
• L-arginine biosynthesis IV (archaea) (3/9 steps found)
• allantoin degradation IV (anaerobic) (2/9 steps found)
• guadinomine B biosynthesis (2/13 steps found)

KEGG Metabolic Maps

• Glutamate metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.5

Use Curated BLAST to search for 6.3.5.5

Protein Sequence (1075 amino acids)

>HMPREF1181_RS07380 carbamoyl-phosphate synthase (glutamine-hydrolyzing) large subunit (Bacteroides stercoris CC31F)
MEKNLKKVLVLGSGALKIGQAGEFDYSGSQALKALREEGVGSVLVNPNIATIQTSEGIAD
KVYFLPVTPYFVEEIIKKEQPDGILLAFGGQTALNCGTELYLNGTLQKYGVRVLGTSVEA
IMYTEDRDLFVKKLDEIPMKTPKSHAVESMEDALKAAREIGYPVMVRSAYALGGLGSGIC
PDEEAFVKLAESSFTFSKQILVEESLKGWKEIEFEVIRDANDHCFTVASMENFDPLGIHT
GESIVVAPTCSLTDEQVKMLQELSTKCIRHLGIVGECNIQYAFNAETNDYRVIEVNARLS
RSSALASKATGYPLAFVAAKIALGYTLDQIGEMGTPNSAYEAPQLDYLICKIPRWDLTKF
AGVSREIGSSMKSVGEIMSIGRSFEEIIQKGLRMIGQGMHGFVGNDSVEFEDLDHELAHP
TDLRVFAIAQALEKGYTIDRIFELTKIDPWFLGKLKNIVDYKNKLSQYNKVEDIPADVLR
EAKVLGFSDFQIARFVLNPEGNMEKENLMVRARRKELGILPAVKRINTVASEHPELTNYL
YMTYAVQGYDVNYYKNEKSVVVLGSGAYRIGSSVEFDWCSVNAIQTARKLGYKSIMINYN
PETVSTDYDMCDRLYFDELSFERVLDVIDLEQPRGVIVSVGGQIPNNLAMKLYRQSVPVL
GTSPVSIDRAENRNKFSAMLDQLGIDQPAWQELTSLEDVKGFVEKVGYPVLVRPSYVLSG
AAMNVCYDEEELENFLKMAAEVSKEYPVVVSQFLQNTKEIEFDAVAQNGEVVEYAISEHV
EFAGVHSGDATLVFPAQKIYFATARRIKKISRQIAKELNISGPFNIQFLARNNEVKVIEC
NLRASRSFPFVSKVLKRNFIETATKIMLDAPYSRPDKSAFDIDWIGVKASQFSFSRLHNA
DPVLGVDMSSTGEVGCIGDDFSEALLNSMIATGFKIPRPEKGVMFSSGAMKSKVDLLDAS
RMLFAKGYKIYATAGTAAFLNAHGVSTEAVFWPDERADAENNVMTMIAEHKFDLIVNIPK
NHSKRELTNGYKIRRGAIDHNIPLITNARLAGAFIEAFCEMKMEDIQIKSWQEYK