Protein Info for HGI48_RS14225 in Dickeya dianthicola 67-19

Annotation: hydrogenase 2 operon protein HybA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 338 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details transmembrane" amino acids 298 to 316 (19 residues), see Phobius details PF12838: Fer4_7" amino acids 46 to 133 (88 residues), 27.1 bits, see alignment E=1.7e-09 amino acids 116 to 164 (49 residues), 29.7 bits, see alignment 2.6e-10 PF13247: Fer4_11" amino acids 111 to 211 (101 residues), 77.4 bits, see alignment E=3e-25 PF12837: Fer4_6" amino acids 142 to 164 (23 residues), 26.8 bits, see alignment (E = 1.3e-09) PF12797: Fer4_2" amino acids 142 to 162 (21 residues), 25.2 bits, see alignment (E = 4e-09) PF00037: Fer4" amino acids 144 to 165 (22 residues), 24.5 bits, see alignment (E = 6.2e-09) PF13187: Fer4_9" amino acids 150 to 206 (57 residues), 27.2 bits, see alignment E=1.1e-09

Best Hits

Swiss-Prot: 74% identical to HYBA_SHIFL: Hydrogenase-2 operon protein HybA (hybA) from Shigella flexneri

KEGG orthology group: None (inferred from 96% identity to ddd:Dda3937_01814)

MetaCyc: 74% identical to hydrogenase 2 iron-sulfur protein (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Fe-S-cluster-containing hydrogenase components 1" in subsystem Anaerobic respiratory reductases or Hydrogenases

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A7L9RF94 at UniProt or InterPro

Protein Sequence (338 amino acids)

>HGI48_RS14225 hydrogenase 2 operon protein HybA (Dickeya dianthicola 67-19)
MNRRNFFKLASAGALLAGTPLATEAAATNRPPIPGSLGMLYDSTLCVGCQACVSKCQQLN
KLDHKPDSAVYAGGAATWSNNDKLSPYTNNIIQVWRSGEGKNKDQAKDGYAYIKKQCMHC
VDPNCVSVCPVSALKKDPKTGVVHYDASVCTGCRYCMVACPFNVPKYDYENPLGKLHKCE
LCNQPELARLDNGGMPGCVEVCPTGAVIYGTREQLLEEAKRRLALKPGDAYAYPRQTLES
KDTYLHTVPSYERYVYGEKEAGGTQVLVLAGVPYQNLEMPTLDSLSTGARSEHIQHTLYK
GMVLPLAVLAGLSVLVHRNTRAEREETHDSQEKPHDGA