Protein Info for HGI48_RS08665 in Dickeya dianthicola 67-19

Annotation: glycoside hydrolase family 3 protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 658 signal peptide" amino acids 1 to 30 (30 residues), see Phobius details PF00933: Glyco_hydro_3" amino acids 106 to 446 (341 residues), 144.6 bits, see alignment E=2.5e-46

Best Hits

Swiss-Prot: 92% identical to BGLX_DICCH: Periplasmic beta-glucosidase/beta-xylosidase (bgxA) from Dickeya chrysanthemi

KEGG orthology group: K05349, beta-glucosidase [EC: 3.2.1.21] (inferred from 94% identity to ddd:Dda3937_03745)

Predicted SEED Role

"Beta-hexosaminidase (EC 3.2.1.52)" in subsystem Chitin and N-acetylglucosamine utilization or N-Acetyl-Galactosamine and Galactosamine Utilization (EC 3.2.1.52)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.21, 3.2.1.52

Use Curated BLAST to search for 3.2.1.21 or 3.2.1.52

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A7L9RCF2 at UniProt or InterPro

Protein Sequence (658 amino acids)

>HGI48_RS08665 glycoside hydrolase family 3 protein (Dickeya dianthicola 67-19)
MTSRYAPHAAVVLPWVATLPLLFSPLAEALQQATLDTREAPLITVGGLKFKDLNRDGKLN
PYEDWRLPPAQRAADLVSRMTLAEKAGVMMHGSAPTAGSVTGAGNQYDLKAANVMIAERH
VNSVITRLSGDNPAQMAEENNKLQQLAENTRLGIPATISTDPRNSFQSLVGVSVSVGKFS
KWPETLGLAAIGDEERVRRFADIVRQEYRAVGITEALSPQADLSTEPRWPRIDGTFGEDP
DLTKKMVRGYVTGMQNGKNGLNGQSVISVVKHWVGYGAAQDGWDSHNAYGKYARFRQNNL
QWHIDPFTGAFEARAAGIMPTYSILRNARWHGKPIEPVGAGFNRFLLTDLLRGQYGFDGV
ILSDWLITNDCKGDCLTGVKPGEKPLPRGMPWGVENLTPAERFIKAVNAGVDQFGGVTDS
GLLVKAVQDGLLSEARLDTSVNRILKQKFQTGLFERPYVNAAQANDIVGRADWQQLADDS
QARALVLLQNNNLLPLRKGSRVWLHGIEANAAQAAGFIVVDAPEQAGVALIRTHTPYEQP
HKNFFFGSRHHEGSLAFRADNPDYQAIVRASARVPTLVTVYMERPAILTNVVGKTQALVA
NFGVSDSVLLNRLISGAAYTAKLPFELPSSMQAVLKQQPDLPYDSDKPLFPFGYGLPH