Protein Info for HGI48_RS07240 in Dickeya dianthicola 67-19

Annotation: beta-galactosidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1037 signal peptide" amino acids 1 to 17 (17 residues), see Phobius details PF02837: Glyco_hydro_2_N" amino acids 62 to 226 (165 residues), 216.4 bits, see alignment E=6.1e-68 PF00703: Glyco_hydro_2" amino acids 228 to 343 (116 residues), 48.5 bits, see alignment E=3.3e-16 PF02836: Glyco_hydro_2_C" amino acids 345 to 637 (293 residues), 388.8 bits, see alignment E=4.1e-120 PF16353: LacZ_4" amino acids 648 to 736 (89 residues), 73.9 bits, see alignment E=2.6e-24 PF02929: Bgal_small_N" amino acids 767 to 1034 (268 residues), 190.3 bits, see alignment E=1.2e-59

Best Hits

Swiss-Prot: 63% identical to BGAL1_KLEP7: Beta-galactosidase 1 (lacZ1) from Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)

KEGG orthology group: K01190, beta-galactosidase [EC: 3.2.1.23] (inferred from 93% identity to ddd:Dda3937_00746)

Predicted SEED Role

"Beta-galactosidase (EC 3.2.1.23)" in subsystem Galactosylceramide and Sulfatide metabolism or Lactose and Galactose Uptake and Utilization or Lactose utilization (EC 3.2.1.23)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.23

Use Curated BLAST to search for 3.2.1.23

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A7L9RBS1 at UniProt or InterPro

Protein Sequence (1037 amino acids)

>HGI48_RS07240 beta-galactosidase (Dickeya dianthicola 67-19)
MSTASAYVPLSGISLADILARRDWENPACPHARRLDAHPPFSSWRSLDAARDDQPSDRRQ
WLNGAWTFSYFPRPEAVPEQWLRQDLTDADSIAVPSNWQLAGYDAPIYTNIKYPIPVNPP
FVPADNPTGCYSLTFTANTDWLVQGQTRIVFDGVNSAFHLWCNGKWVGYSQDSRLPAEFD
LTSCLQPGENRLAVMVLRWSDGTYLEDQDMWRMSGIYRDVYLLHKPAVHLRDVQITTPLR
HSYTQGTLCVTAQANLPEEQAQAWQLYVQLWRGEQWVGERRAPFGTPVIDERGAYHDRVS
LQLEVERPALWSAEEPNLYRAVVALEHADGTLVEAEAYDVGFREVAIRNGLLLLNGQPLL
IRGVNRHEHHPQRGQAIDEATMRQDILLMKRHNFNAVRCSHYPNHPLWYRLCDRYGLYVV
DEANIETHGMQPMSRLSDDPRWLPAYAERVTRMVQRDRNHPSIIIWSLGNESGYGPTHSA
LYQWVKQQDPSRPVQYEGGGANTPATDILCPMYARVDQDQPFPAVPKWSIKKWIGLPGEN
RPLILCEYAHAMGNSVGGFDRYWQAFRQYPRLQGGFVWDWVDQALVREQEGKPHWAYGGD
FGDKPNDRQFCLNGLVFPDRTPHPALYEAQRAQQFFQFSHHENHPLTLTVTSEYLFRHSD
NEELRWRIMQDDVQLASGVVPLTVAPQGCQTMTLLDRLPAPQHRADMWLTVEVVQPNATE
WSPAGHRCAWDQWPLPMPLARTTPRRDDGACPTLTQDDDRFDIVHGQQRWNFDRRSGLLT
QWWRDGQPQLLSPLHDNLARAPLDNDIGISEVDRIDPNAWVERWKLAGLYQYDTDCRQIH
ADTLSDSVLITTEHVGHYQQQVLFISRKQWRIDAQGALTVSVDVEVARHLPPLARIGLCG
QLAAVNPQVSWLGLGPHENYPDRRLAALHGRWQQPLEAMHTPYIFPSENGLRCHTRELRY
GHWLIEGDFHFGIGRYSLRQLMDCTHQHLLQPEPGTWLNLDGFHMGIGGDDSWSPSVAPD
FLLTAPRYRYQLQLRLQ