Protein Info for HER17_RS21130 in Pectobacterium carotovorum WPP14

Annotation: mannitol-1-phosphate 5-dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01232: Mannitol_dh
  • amino acids 1 to 123 (123 residues), 87.3 bits, E=1.3e-28
• PF08125: Mannitol_dh_C
  • amino acids 148 to 372 (225 residues), 217.2 bits, E=2.6e-68

Best Hits

Swiss-Prot: 98% identical to MTLD_PECCP: Mannitol-1-phosphate 5-dehydrogenase (mtlD) from Pectobacterium carotovorum subsp. carotovorum (strain PC1)

KEGG orthology group: K00009, mannitol-1-phosphate 5-dehydrogenase [EC: 1.1.1.17] (inferred from 98% identity to pwa:Pecwa_4446)

MetaCyc: 78% identical to mannitol-1-phosphate 5-dehydrogenase (Escherichia coli K-12 substr. MG1655)
Mannitol-1-phosphate 5-dehydrogenase. [EC: 1.1.1.17]

Predicted SEED Role

"Mannitol-1-phosphate 5-dehydrogenase (EC 1.1.1.17)" in subsystem Mannitol Utilization (EC 1.1.1.17)

MetaCyc Pathways

• hexitol fermentation to lactate, formate, ethanol and acetate (17/19 steps found)
• superpathway of hexitol degradation (bacteria) (15/18 steps found)
• mannitol cycle (4/5 steps found)
• mannitol degradation I (1/1 steps found)

KEGG Metabolic Maps

• Fructose and mannose metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.17

Protein Sequence (382 amino acids)

>HER17_RS21130 mannitol-1-phosphate 5-dehydrogenase (Pectobacterium carotovorum WPP14)
MKALHFGAGNIGRGFIGKLLADANVELTFADVNQPLLDALNSRTSYTVRIVGDNTQVDTV
SNVSAVHSGSQDAVALIAVADLVTTAVGPQILEKIAGTIAQGLVKRHNDGNTRPLNIIAC
ENMVRGTSQLKQHVLKLLPEGHQEWVVEHVGFVDSAVDRIVPPSEAGSDDVLAVTVETFS
EWIVDKTQFCGEPPAIPGMELTDNLMAFVERKLFTLNTGHAITAYLGQQARHQTIRDAIL
DPKVRAVVKGAMEESGAVLIKRYGFDAEKHAAYINKILSRFENPHLHDDVERVGRQPLRK
LSAGDRLIKPLLGTLEYQLPHDNLITGIAAAMHYRSEQDPQALELAELIRTQGPQAALVQ
ISGLDADSEVVAQAVNVYNAMQ