Protein Info for HER17_RS20765 in Pectobacterium carotovorum WPP14

Annotation: glycine C-acetyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 398 TIGR01822: glycine C-acetyltransferase" amino acids 5 to 397 (393 residues), 682.8 bits, see alignment E=6.7e-210 PF00155: Aminotran_1_2" amino acids 44 to 387 (344 residues), 242.4 bits, see alignment E=8.7e-76 PF01041: DegT_DnrJ_EryC1" amino acids 89 to 220 (132 residues), 23.9 bits, see alignment E=2.4e-09

Best Hits

Swiss-Prot: 79% identical to KBL_SHIFL: 2-amino-3-ketobutyrate coenzyme A ligase (kbl) from Shigella flexneri

KEGG orthology group: K00639, glycine C-acetyltransferase [EC: 2.3.1.29] (inferred from 96% identity to eca:ECA0167)

MetaCyc: 79% identical to 2-amino-3-ketobutyrate CoA ligase (Escherichia coli K-12 substr. MG1655)
Glycine C-acetyltransferase. [EC: 2.3.1.29]

Predicted SEED Role

"2-amino-3-ketobutyrate coenzyme A ligase (EC 2.3.1.29)" in subsystem Glycine Biosynthesis or Glycine and Serine Utilization (EC 2.3.1.29)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.29

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (398 amino acids)

>HER17_RS20765 glycine C-acetyltransferase (Pectobacterium carotovorum WPP14)
MPAAFYQQLTTQVMAARTEGVFKEERIITSAQQAEIEVMDSDRLLNFCANNYLGLADSPE
LIAAAKAGLDSHGFGMASVRFICGTQDIHKQLERKLADFLGMDDAILYSSCFDANGGLFE
TLMGPEDAIISDALNHASIIDGIRLSKARRYRYANNDMSQLEAQLQLARAEGARHVMIAT
DGVFSMDGVIADLQGICDLADRYDALVMVDDSHAVGFVGEQGRGTHERCGVMNRVDIITG
TLGKALGGASGGYTAGKHEVIDWLRQRSRPYLFSNSLAPAIVTASLRVLDLLEQGGERRE
RLWANARLFREKMTAAGFTLAGADHAIIPVMLGEAQLAQDFAQALQREGVYVAGFFYPVV
PLGQARIRTQMSAAHTPQQIEFAVEAFIRVGKRLGVVI