Protein Info for HER17_RS19355 in Pectobacterium carotovorum WPP14
Annotation: aspartate ammonia-lyase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 90% identical to ASPA_SERMA: Aspartate ammonia-lyase (aspA) from Serratia marcescens
KEGG orthology group: K01744, aspartate ammonia-lyase [EC: 4.3.1.1] (inferred from 99% identity to pwa:Pecwa_0480)MetaCyc: 88% identical to aspartate ammonia-lyase (Escherichia coli K-12 substr. MG1655)
Aspartate ammonia-lyase. [EC: 4.3.1.1]
Predicted SEED Role
"Aspartate ammonia-lyase (EC 4.3.1.1)" in subsystem Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 4.3.1.1)
MetaCyc Pathways
- superpathway of L-aspartate and L-asparagine biosynthesis (4/4 steps found)
- L-aspartate degradation II (aerobic) (3/3 steps found)
- L-aspartate degradation III (anaerobic) (3/3 steps found)
- L-glutamate degradation II (2/2 steps found)
- L-canavanine degradation II (1/6 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 4.3.1.1
Use Curated BLAST to search for 4.3.1.1
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (479 amino acids)
>HER17_RS19355 aspartate ammonia-lyase (Pectobacterium carotovorum WPP14) MSTNIRIEEDLLGTREVPADAYYGIHTLRAIENFYISNSTISDIPEFVRAMVMVKKAAAL ANKELQTIPRKIADTILQACDEVLNNGKCLDQFPVDVYQGGAGTSVNMNTNEVLANIGLE LMGHQKGEYQYLNPNDHLNKCQSTNDAYPTGFRIAVYTSILKLVEAITQLSDGFERKAKE FENILKMGRTQLQDAVPMTLGQEFHAFNVLLKEENRNLLRTAELLLEVNLGATAIGTRLN TPDEYQKLAVQHLAKVSGLPCVPAEDLIEATSDCGAYVMMHSALKRVAVKMSKICNDLRL LSSGPRTGLNEINLPELQAGSSIMPAKVNPVVPEVVNQVCFKVIGNDTCVTMAAEAGQLQ LNVMEPVIGQAMFESIQILSNACYNLLEKCVDGITANKEVCEAYVFNSIGIVTYLNPFIG HHNGDIVGKICAETGKSVREVVLERGLLTEEQLDDIFSIQNLMHPAYKAKRYTDENEAV