Protein Info for HER17_RS18215 in Pectobacterium carotovorum WPP14

Annotation: transcription termination factor NusA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 509 PF08529: NusA_N" amino acids 4 to 123 (120 residues), 133 bits, see alignment E=9.2e-43 TIGR01953: transcription termination factor NusA" amino acids 4 to 353 (350 residues), 410.9 bits, see alignment E=3.9e-127 PF13184: KH_5" amino acids 236 to 303 (68 residues), 98.9 bits, see alignment E=2.2e-32 TIGR01954: transcription termination factor NusA, C-terminal duplication" amino acids 378 to 427 (50 residues), 75.2 bits, see alignment 3.3e-25 amino acids 454 to 502 (49 residues), 60.3 bits, see alignment 1.5e-20 PF14520: HHH_5" amino acids 381 to 424 (44 residues), 22.3 bits, see alignment 2.3e-08 amino acids 445 to 499 (55 residues), 39.2 bits, see alignment 1.2e-13

Best Hits

Swiss-Prot: 85% identical to NUSA_SALTY: Transcription termination/antitermination protein NusA (nusA) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K02600, N utilization substance protein A (inferred from 99% identity to pct:PC1_0584)

Predicted SEED Role

"Transcription termination protein NusA" in subsystem NusA-TFII Cluster or Transcription factors bacterial

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (509 amino acids)

>HER17_RS18215 transcription termination factor NusA (Pectobacterium carotovorum WPP14)
MNKEILAVVEAVSNEKALPREKIFEALETALATATKKKYEQEIDVRVSIDRKTGDFDTFR
RWLVVNEVTQPTREITLEAAQFEDPALDVGGYVEDQIESVTFDRITTQTAKQVIVQKVRE
AERAMVVDQFREQEGEIITGVVKKVNRDNISLEVRPIDGSNTSAEAVIGREDMLPRENFR
PGDRIRGVLYSVRPEARGAQLFVSRSRPEMLVELFRIEVPEIGEEVIEIKAAARDPGSRA
KIAVKTNDKRIDPVGACVGMRGARVQAVSSELGGERIDIILWDDNPAQFVINAMAPADVV
SIVVDEDTCTMDIAVESSNLAQAIGRNGQNVRLASQLLKQHRSDDRWELNVMTVEDLQAK
HQAEAHAAIDVFTKHLDIDEEFATVLVEEGFSSLEELAYVPIKELLAIEGLDEETVEALR
ERAKAALTTLALAHEESLGDGQPAEDLLGLPGLSRELAFKLAAIGVCTLEDLAEQGVDDL
TDIEELNDEQAGELIMAARNICWFGDDNE