Protein Info for HER17_RS17135 in Pectobacterium carotovorum WPP14

Annotation: NADH:flavorubredoxin reductase NorW

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 382 PF07992: Pyr_redox_2" amino acids 2 to 274 (273 residues), 197.5 bits, see alignment E=1e-61 PF13738: Pyr_redox_3" amino acids 65 to 271 (207 residues), 52.6 bits, see alignment E=1.3e-17 PF00070: Pyr_redox" amino acids 142 to 216 (75 residues), 58.4 bits, see alignment E=2.6e-19 PF18113: Rbx_binding" amino acids 305 to 374 (70 residues), 72 bits, see alignment E=8.4e-24

Best Hits

Swiss-Prot: 92% identical to NORW_PECAS: Nitric oxide reductase FlRd-NAD(+) reductase (norW) from Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672)

KEGG orthology group: K12265, nitric oxide reductase FlRd-NAD(+) reductase [EC: 1.18.1.-] (inferred from 92% identity to pwa:Pecwa_1020)

MetaCyc: 60% identical to NADH:flavorubredoxin reductase (Escherichia coli K-12 substr. MG1655)
Rubredoxin--NAD(+) reductase. [EC: 1.18.1.1]

Predicted SEED Role

"Nitric oxide reductase FlRd-NAD(+) reductase (EC 1.18.1.-)" in subsystem Nitrosative stress (EC 1.18.1.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.18.1.- or 1.18.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (382 amino acids)

>HER17_RS17135 NADH:flavorubredoxin reductase NorW (Pectobacterium carotovorum WPP14)
MKDIVIIGAGFAARQLIRQLRKQDAHSPIRLITADSGDEYNKPDLSHVMSLQQHADDLTK
LSAAAFAEENRITLLANTRVTAIDRHTQQVICGTDRYDYHKLVFATGASAMVPPIPGREH
MLTLNSQQEYRAHEGRLWQAERVLVLGAGLIGTELAMDLGRAGKQVTLVDCANSILPALM
PPEVSARLQFTLTQQGVSLQLNTTLQQLEKTETGVQATFTDGRSVDVDEILSAIGLQANT
QLAKAAGLAVQKGIQTNAQLQTTDPQIYALGDCAEIGGKLLPFLQPIQLSAITLAKNLLG
ASETLTLPPMLVKIKTPLFPLQMAGDTTGSDLHWQQEWNEQGMVAKALDGQQMLRAFVVG
GERMKEAFPLLRQLSTVSSTTA