Protein Info for HER17_RS16015 in Pectobacterium carotovorum WPP14

Annotation: proline-specific permease ProY

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 458 transmembrane" amino acids 19 to 38 (20 residues), see Phobius details amino acids 45 to 63 (19 residues), see Phobius details amino acids 89 to 118 (30 residues), see Phobius details amino acids 125 to 143 (19 residues), see Phobius details amino acids 155 to 176 (22 residues), see Phobius details amino acids 197 to 220 (24 residues), see Phobius details amino acids 241 to 261 (21 residues), see Phobius details amino acids 279 to 299 (21 residues), see Phobius details amino acids 331 to 351 (21 residues), see Phobius details amino acids 357 to 379 (23 residues), see Phobius details amino acids 399 to 422 (24 residues), see Phobius details amino acids 428 to 446 (19 residues), see Phobius details PF03845: Spore_permease" amino acids 12 to 281 (270 residues), 27.8 bits, see alignment E=1.8e-10 PF00324: AA_permease" amino acids 16 to 452 (437 residues), 394.1 bits, see alignment E=1.3e-121 PF13520: AA_permease_2" amino acids 17 to 440 (424 residues), 135.2 bits, see alignment E=4.8e-43

Best Hits

Swiss-Prot: 84% identical to PROY_SALTY: Proline-specific permease ProY (proY) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K03293, amino acid transporter, AAT family (inferred from 99% identity to pwa:Pecwa_3297)

MetaCyc: 48% identical to threonine/serine:H+ symporter ThrP (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-71; TRANS-RXN-72

Predicted SEED Role

"Proline-specific permease proY" in subsystem Proline, 4-hydroxyproline uptake and utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (458 amino acids)

>HER17_RS16015 proline-specific permease ProY (Pectobacterium carotovorum WPP14)
MEQPSSKLKRGLSTRHIRFIALGSAIGTGLFYGSASAIQMAGPSVLLAYLIGGVVAYIIM
RALGEMSVHNPQSSSFSRYAQDYLGPLAGYITGWTYCFEMLIVAIADVTAFGIYMGVWFP
AVPHWVWVLSVVLIIGAINLMNVKAFGELEFWLSFFKVATIIIMIAAGIGIIIWGIGNGG
EPTGIHNLWSNGGFFSNGVMGMILSLQLVMFAYGGVEIIGITAGEAKDPQKSIPRAINSV
PWRILVFYVGTLFVIMSIYPWNQVGTNGSPFVLTFQHMGITAAAGILNFVVITASLSAIN
SDVFGVGRMLHGMAEQGHAPKVFSRISKRGIPWVTVVVMMMALLVAVYLNYIMPGKVFLV
IASLATFATVWVWIMILFSQIAFRRRLSPDEVKALAFPLRGGIVTSVFGIVFLFFIIGLI
GYFPDTRVSLYVGIIWILLLLVGYVWKKKRQNAVAAQN