Protein Info for HER17_RS15910 in Pectobacterium carotovorum WPP14

Annotation: 2-dehydropantoate 2-reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 302 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details TIGR00745: 2-dehydropantoate 2-reductase" amino acids 2 to 289 (288 residues), 254.8 bits, see alignment E=5.5e-80 PF02558: ApbA" amino acids 3 to 143 (141 residues), 94.8 bits, see alignment E=4.1e-31 PF08546: ApbA_C" amino acids 167 to 288 (122 residues), 110.5 bits, see alignment E=7.3e-36

Best Hits

Swiss-Prot: 69% identical to PANE_YERPE: 2-dehydropantoate 2-reductase (panE) from Yersinia pestis

KEGG orthology group: K00077, 2-dehydropantoate 2-reductase [EC: 1.1.1.169] (inferred from 98% identity to pct:PC1_1035)

MetaCyc: 63% identical to 2-dehydropantoate 2-reductase (Escherichia coli K-12 substr. MG1655)
2-dehydropantoate 2-reductase. [EC: 1.1.1.169]

Predicted SEED Role

"2-dehydropantoate 2-reductase (EC 1.1.1.169)" in subsystem Coenzyme A Biosynthesis (EC 1.1.1.169)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.169

Use Curated BLAST to search for 1.1.1.169

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (302 amino acids)

>HER17_RS15910 2-dehydropantoate 2-reductase (Pectobacterium carotovorum WPP14)
MKITVLGCGALGQLWLAALHQQGHDVQGWLRIPQPYCPVNVTLLDGQHCNLNLTANDPDH
LAQSELLLVTLKAWQVSDAVTVLLPQLNPHCTILLLHNGMGTREELPSLQQPLVLGITTH
AARRDANNVVHVAAGTTHIGTFNGDSSQSHLAEVLHQALPDVAWHTNISATCWLKLAANC
VINPLTVKYHCTNGELSAYPELIASLCQEVTSVMEREGFHTSRDSLLLYVNQIIQNTAAN
TSSMLQDILAQRHTEIDYITGYLLHRARLHGLTLPENARLFDYIKQKENDYDRIGTGVSG
TW