Protein Info for HER17_RS14535 in Pectobacterium carotovorum WPP14

Annotation: GDP-L-fucose synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01370: Epimerase
  • amino acids 5 to 229 (225 residues), 216.3 bits, E=6.6e-68
• PF16363: GDP_Man_Dehyd
  • amino acids 37 to 304 (268 residues), 66.9 bits, E=3.5e-22

Best Hits

Swiss-Prot: 76% identical to FCL_ECOLI: GDP-L-fucose synthase (fcl) from Escherichia coli (strain K12)

KEGG orthology group: K02377, GDP-L-fucose synthase [EC: 1.1.1.271] (inferred from 94% identity to pct:PC1_1313)

MetaCyc: 76% identical to GDP-L-fucose synthase (Escherichia coli K-12 substr. MG1655)
GDP-L-fucose synthase. [EC: 1.1.1.271]

Predicted SEED Role

"GDP-L-fucose synthetase (EC 1.1.1.271)" in subsystem Capsular heptose biosynthesis or Colanic acid biosynthesis (EC 1.1.1.271)

MetaCyc Pathways

• colanic acid building blocks biosynthesis (11/11 steps found)
• GDP-L-fucose biosynthesis I (from GDP-D-mannose) (3/3 steps found)
• superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis (9/14 steps found)

KEGG Metabolic Maps

• Fructose and mannose metabolism
• Nucleotide sugars metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.271

Protein Sequence (320 amino acids)

>HER17_RS14535 GDP-L-fucose synthase (Pectobacterium carotovorum WPP14)
MKKRVYVAGHRGMVGSAIVRQLQTRNDIEVVVRTRDELDLLSQDAVKSFFANEHIDEVYL
AAAKVGGIHANNTYPAEFIYENLMIESNIINYAHQSGVHKLLFLGSSCIYPKLVEQPMKE
SALLTGILESTNEPYAIAKIAGIKLCESYNRQYGRDYRSVMPTNLYGENDNFHPENSHVI
PALMRRFHEAKVLNSPEVVVWGSGKPMREFLFVDDMASASIHVMELDKNVYDSHTDPMLS
HINVGTGVDCTIGEMANTMAKVVGYQGQVVFDASKPDGTPRKLMDVSRLKKLGWQYKVEL
EEGLLKTYHWFLANQNSFRK